eNOS Antibody


Western Blot: eNOS Antibody [NBP1-19867] - Extracts from COLO cells.

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eNOS Antibody Summary

Synthetic peptide, corresponding to amino acids 455-500 of Human NOS3.
Immunogen affinity purified
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  • Western Blot 1:500-1:1000
Application Notes
Western blot (WB) analyzes of NOS3 (G488) pAb in extracts from COLO cells.
Theoretical MW
140 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Human, Mouse, Rat

Packaging, Storage & Formulations

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
PBS (approx. pH 7.2)
0.05% Sodium Azide
1.0 mg/ml
Immunogen affinity purified

Alternate Names for eNOS Antibody

  • Constitutive NOS
  • EC
  • Endothelial NOS
  • endothelial
  • eNOS
  • eNOScNOS
  • nitric oxide synthase 3 (endothelial cell)
  • NOS type III
  • NOS3


Nitric oxide synthase NOS oxidizes a guanidine nitrogen of arginine releasing nitric oxide in the form of a free radical and citrulline. Nitric oxide thus generated acts as a messenger in diverse functions including vasodilation neurotransmission, anti tumor and anti pathogenic activities. NOS is classified under three types: neuronal NOS (nNOS) or brain NOS (bNOS); inducible NOS (iNOS) or macrophage NOS (mNOS); and endothelial NOS (eNOS).eNOS is a calcium/calmodulin dependent enzyme which undergoes several post translational modifications, including acylation with myristate and palmitate, and phosphorylation on numerous residues. As with the other members of the NOS family, eNOS derives the diffusible multifunctional second messenger NO from L arginine through a series of reactions in which L citrulline is an intermediate. eNOS plays an important role in controlling vascular tone, platelet aggregation, and cardiac myocyte function.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

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Secondary Antibodies


Isotype Controls

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Gene Symbol NOS3