Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
PBS containing 5% trehalose.
Protein G purified
ELISA: Use at a concentration of 0.5 - 1 ug/ml. The detection limit for recombinant mouse endostatin is approximately 6 ng/well. WB: Use at a concentration of 1 - 2 ug/ml. The detection limit for recombinant mouse endostatin is approximately 50 ng/lane and 5 ng/lane under non-reducing and reducing conditions, respectively. Predicted molecular weight: 22 kDa.
Read Publication using NB120-10604 in the following applications:
Endostatin (20-22 kDa), a cleaved product of the C-terminal domain of collagen XVIII, is an endogenous angiogenesis inhibitor. It was originally found in conditioned media from a murine endothelial tumor cell line, hemangioendothelioma. Endostatin inhibits endothelial cell migration (in vivo and in vitro) and induces endothelial cell apoptosis. It inhibits tumor growth and impairs blood vessel maturation in wound healing. Endostatin has an important role in endothelial cell adhesion and cytoskeletal organization. Endostatin can be found in vessel walls (elastic fibers) and basement membranes.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.