Human Endoglin/CD105 ELISA Kit

Product Discontinued

Human Endoglin/CD105 ELISA Kit Summary

• Marker: Hematopoietic Stem Cell Marker
• Specificity: Human Endoglin/CD105
• Kit Type: ELISA Kit
• Gene: ENG

Applications/Dilutions

• Dilutions:
  • ELISA 156pg/ml-10000pg/ml
• Application Notes: This product is useful for Sandwich ELISA detection. Strip format Biosensis ELISA kits offer the highest level of sensitivity available Strip 96-well format, use only what you need: High sensitivity and low blank Suitability for serum & tissues Reproducible results This kit contains: Pre-coated plates, Prepared standards Buffers, Detection reagents, Substrate Precise methods/instructions.

Reactivity Notes

Human CD105 protein in a sandwich ELISA

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.

Notes

This kit contains 2.0 x 96.0 Well Strip.

Alternate Names for Human Endoglin/CD105 ELISA Kit

• CD105 antigen
• CD105
• Endoglin
• ENDOsler-Rendu-Weber syndrome 1
• ENG
• HHT1FLJ41744
• ORW
• ORW1

Background

The human Endoglin/CD105 Kit is a sandwich ELISA kit. The capture antibody is a monoclonal human Endoglin/CD105 antibody (clone No. 166713) pre-coated onto the 96-well strip plates provided in the kit. Human test samples and standards of known Endoglin/CD105 concentration are added to these wells and allowed to complex with the bound Endoglin/CD105 antibody. A biotinylated human Endoglin/CD105 polyclonal antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of Endoglin/CD105 present in the samples. The purpose of this kit is the in-vitro quantitative determination of human Endoglin/CD105 in samples such as sera, plasma, tissue lysates and cell culture supernates. This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.



This product is distributed on behalf of Biosensis Pty. Ltd. of Australia.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

FAQs for Endoglin/CD105 ELISA Kit (BEK-2153-2P). (Showing 1 - 3 of 3 FAQ).

1. I wonder if you have a CD105 or CD34 antibody suitable for IHC that is specific for human and do not bind mouse?
   • We do not have any anti-human CD34 or CD105 antibodies that are confirmed to NOT detect the mouse protein. When we have tested an antibody and confirmed that it will not react with mouse samples, we will add Mu(-) to the datasheet, and unfortunately all of our CD105 and CD34 antibodies will either detect the mouse protein, or they have not been used in mouse samples before.
2. We are interested in CD105 antibodies that could react with dog, please let me know which products in your catalog you would recommend.
   • We do not currently carry any CD105 antibodies that have been validated for use in dog samples. However, if you are interested in testing any of our CD105 antibodies with dog samples you would be eligible for our Innovators Reward Program, in which you can get a discount voucher for the purchase price of the product. Please contact us at innovators@novusbio.com with any questions regarding this program.
3. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: ENG