Endo G Antibody (7G1C10)

Images

 
Western Blot: Endo G Antibody (7G1C10) [NBP1-75500] - analysis of EndoG expression in HepG2 cell lysate with EndoG antibody at (A) 2.5 and (B) 5 ug/mL.

Product Details

Summary
Product Discontinued
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Order Details


    • Catalog Number
      NBP1-75500
    • Availability
      Product Discontinued

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Endo G Antibody (7G1C10) Summary

Immunogen
Recombinant protein corresponding to amino acids 51 - 140 of human EndoG.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
ENDOG
Purity
Immunogen affinity purified
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Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • ELISA 1:100-1:2000
Application Notes
EndoG monoclonal antibody can be used for detection of EndoG by Western blot at 2.5 - 5 g/mL.

Reactivity Notes

Human, Mouse, Rat. Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Endo G Antibody (7G1C10)

  • EC 3.1.30.-
  • Endo G
  • endonuclease G
  • endonuclease G, mitochondrial
  • FLJ27463
  • mitochondrial endonuclease G

Background

The fragmentation of nuclear DNA is a hallmark of apoptotic cell death. The activities of caspase and nuclease are involved in the DNA fragmentation. Caspase-activated deoxyribonuclease (CAD), also termed DNA fragmentation factor (DFF40), is one such nuclease, and is capable of inducing DNA fragmentation and chromatin condensation after cleavage by caspase-3 of its inhibitor ICAD/DFF45. Caspase and CAD independent DNA fragmentation also exists. Recent studies demonstrated that another nuclease, endonuclease G (EndoG), is specifically activated by apoptotic stimuli and is able to induce nucleosomal fragmentation of DNA independently of caspase and DFF/CAD. EndoG is a mitochondrion-specific nuclease that translocates to the nucleus and cleaves chromatin DNA during apoptosis. The homologue of mammalian EndoG is the first mitochondrial protein identified to be involved in apoptosis in C. elegans. EndoG also cleaves DNA in vitro.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol ENDOG
Uniprot