Endo G Antibody (5F1)

Images

 
Western Blot: Endo G Antibody (5F1) [NBP2-01696] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Endo G (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates ...read more
Immunocytochemistry/ Immunofluorescence: Endo G Antibody (5F1) [NBP2-01696] Staining of COS7 cells transiently transfected by pCMV6-ENTRY Endo G.
Immunohistochemistry-Paraffin: Endo G Antibody (5F1) [NBP2-01696] - Staining of paraffin-embedded Human tonsil using anti-Endo G mouse monoclonal antibody.
Flow Cytometry: Endo G Antibody (5F1) [NBP2-01696] - Analysis of Jurkat cells, using anti-Endo G antibody, (Red), compared to a nonspecific negative control antibody (Blue).
Immunohistochemistry-Paraffin: Endo G Antibody (5F1) [NBP2-01696] - Staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-Endo G mouse monoclonal antibody.
Immunohistochemistry-Paraffin: Endo G Antibody (5F1) [NBP2-01696] - Staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-Endo G mouse monoclonal antibody.
Immunohistochemistry-Paraffin: Endo G Antibody (5F1) [NBP2-01696] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-Endo G mouse monoclonal antibody.
Immunohistochemistry-Paraffin: Endo G Antibody (5F1) [NBP2-01696] - Staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-Endo G mouse monoclonal antibody.
Immunohistochemistry-Paraffin: Endo G Antibody (5F1) [NBP2-01696] - Staining of paraffin-embedded Carcinoma of Human liver tissue using anti-Endo G mouse monoclonal antibody.
Immunohistochemistry-Paraffin: Endo G Antibody (5F1) [NBP2-01696] - Staining of paraffin-embedded Human Kidney tissue using anti-Endo G mouse monoclonal antibody.
Immunohistochemistry-Paraffin: Endo G Antibody (5F1) [NBP2-01696] - Staining of paraffin-embedded Human liver tissue using anti-Endo G mouse monoclonal antibody.
Immunohistochemistry-Paraffin: Endo G Antibody (5F1) [NBP2-01696] - Staining of paraffin-embedded Human pancreas tissue using anti-Endo G mouse monoclonal antibody.
Flow Cytometry: Endo G Antibody (5F1) [NBP2-01696] - Analysis of Hela cells, using anti-Endo G antibody, (Red), compared to a nonspecific negative control antibody (Blue).

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IHC-P
Clone
5F1
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

Endo G Antibody (5F1) Summary

Immunogen
Full length human recombinant protein of human ENDOG(NP_004426) produced in HEK293T cell.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
ENDOG
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-2000
  • Flow Cytometry 1:100
  • Immunocytochemistry/Immunofluorescence 1:100
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin 1:150

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for Endo G Antibody (5F1)

  • EC 3.1.30.-
  • Endo G
  • endonuclease G
  • endonuclease G, mitochondrial
  • FLJ27463
  • mitochondrial endonuclease G

Background

The protein encoded by this gene is a nuclear encoded endonuclease that is localized in the mitochondrion. The encoded protein is widely distributed among animals and cleaves DNA at GC tracts. This protein is capable of generating the RNA primers required by DNA polymerase gamma to initiate replication of mitochondrial DNA. [provided by RefSeq].

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

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ICC/IF Video Protocol

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Bioinformatics

Gene Symbol ENDOG
Entrez