EMP3 Antibody (439949) [Unconjugated]

Human peripheral blood mononuclear cells were stained with Mouse Anti-Human EMP3 Monoclonal Antibody (Catalog # MAB4505, filled histogram) or isotype control antibody (MAB0041, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG F(ab')2Secondary Antibody (F0102B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.

PBMC monocytes were stained with Mouse Anti-Human EMP3 Monoclonal Antibody (Catalog # MAB4505, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). To facilitate intracellular staining, cells were fixed and permeabilized with Flow Cytometry Fixation Buffer (Catalog # FC004). View our protocol for Staining Intracellular Molecules.

• Reactivity: Hu
• Applications: CyTOF-ready, Flow
• Clone: 439949
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

EMP3 Antibody (439949) [Unconjugated] Summary

• Immunogen: NS0 mouse myeloma cell line transfected with human EMP3
  Met1-Glu163
  Accession # P54852
• Specificity: Detects human EMP. Stains human EMP3 cell transfectants but not irrelevant transfectants.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: EMP3
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Intracellular Staining by Flow Cytometry 0.25 ug/10^6 cells

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for EMP3 Antibody (439949) [Unconjugated]

• EMP3
• EMP-3
• epithelial membrane protein 3
• HNMP-1
• Protein YMP
• YMP
• YMPHematopoietic neural membrane protein 1

Background

Epithelial membrane protein 3 (EMP3) is a multipass transmembrane protein in the peripheral myelin protein 22 family. It is expressed as a 20‑25 kDa molecule depending on the degree of glycosylation. EMP3 interacts with the P2X7 purinergic receptor on monocytes. Its dysregulation is associated with glioma and neuroblastoma. Human EMP3 shares 93% aa sequence identity with mouse and rat EMP3.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for EMP3 Antibody (MAB4505)(1)

Publication using MAB4505

• N Thornton, V Karamatic, L Tilley, CA Green, CL Tay, RE Griffiths, BK Singleton, F Spring, P Walser, AG Alattar, B Jones, R Laundy, JR Storry, M Möller, L Wall, R Charlewood, CM Westhoff, C Lomas-Fran, V Yahalom, U Feick, A Seltsam, B Mayer, ML Olsson, DJ Anstee Disruption of the tumour-associated EMP3 enhances erythroid proliferation and causes the MAM-negative phenotype Nat Commun, 2020-07-16;11(1):3569. 2020-07-16 [PMID: 32678083] (Flow Cytometry, Neutralization, Human)

Bioinformatics

• Gene Symbol: EMP3
• Uniprot:
  • P54852()