EMG1 Antibody - BSA Free

Images

 
Western Blot: EMG1 Antibody [NBP1-57410] - HepG2 cell lysate, recommended antibody concentration: 0.2 - 1 ug/mL.
Immunohistochemistry-Paraffin: EMG1 Antibody [NBP1-57410] - Human kidney tissue. Recommended antibody concentration 4 - 8 ug/mL. Cells with positive label: renal corpuscle cells (indicated with arrows) 400X magnifiation.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Format
BSA Free
Concentration
0.5 mg/ml

Order Details

EMG1 Antibody - BSA Free Summary

Description
The addition of 50% glycerol is optional for those storing this antibody at -20C and not aliquoting smaller units. However, please note that glycerol may interrupt some downstream antibody applications and should be added with caution.
Immunogen
Synthetic peptides corresponding to EMG1 (EMG1 nucleolar protein homolog (S. cerevisiae)). The peptide sequence was selected from the N terminal of EMG1. Peptide sequence SLETVKVGKTYELLNCDKHKSILLKNGRDPGEARPDITHQSLLMLMDSPL The peptide sequence for this immunogen was taken from within the described region.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
EMG1
Purity
Affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:10 - 1:500
  • Immunohistochemistry-Paraffin 1:10 - 1:500
  • Western Blot 1.0 ug/ml
Reviewed Applications
Read 1 Review rated 1
using
NBP1-57410 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS, 2% Sucrose
Preservative
0.09% Sodium Azide
Concentration
0.5 mg/ml
Purity
Affinity purified

Alternate Names for EMG1 Antibody - BSA Free

  • 18S rRNA (pseudouridine-N1-)-methyltransferase NEP1
  • BWCNS
  • C2F18S rRNA Psi1248 methyltransferase
  • EMG1 nucleolar protein homolog (S. cerevisiae)
  • essential for mitotic growth 1
  • FLJ60792
  • Grcc2f
  • NEP1EC 2.1.1.-
  • Nucleolar protein EMG1 homolog
  • probable ribosome biogenesis protein NEP1
  • Protein C2f
  • Ribosome biogenesis protein NEP1

Background

EMG1 involved in 40S ribosomal subunit biogenesis. It seems to play a role a methylation reaction in pre-rRNA processing.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for EMG1 Antibody (NBP1-57410) (0)

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Review for EMG1 Antibody (NBP1-57410) (1) 11

Average Rating: 1
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NBP1-57410:
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WB
(1)
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Images Ratings Applications Species Date Details
Western Blot EMG1 NBP1-57410
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1
reviewed by:
Verified Customer
WB Human 03/25/2019
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Summary

ApplicationWestern Blot
Sample Testedb-cell lymphocyte,B-cell whole cell lysate 40ug
SpeciesHuman
Lotqc10242

Comments

CommentsI used this antibody at a 1:500 dilution (1ug/mL) overnight 4C. I ran 40ug of total cell lysate from B-cell lymphocytes. I also ran the same sample with a second EMG1 antibody (Origene TA501209) which had a very strong signal. The image submitted is a 3 minute exposure. A longer exposure did not reveal any specific bands.

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FAQs for EMG1 Antibody (NBP1-57410) (0)

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Secondary Antibodies

 

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Verified Customer
03/25/2019
Application: WB
Species: Human

Bioinformatics

Gene Symbol EMG1
Uniprot