EMG1 Antibody

Images

 
Western Blot: EMG1 Antibody [NBP1-57410] - HepG2 cell lysate, recommended antibody concentration: 0.2 - 1 ug/mL.
Immunohistochemistry-Paraffin: EMG1 Antibody [NBP1-57410] - Human kidney tissue. Recommended antibody concentration 4 - 8 ug/mL. Cells with positive label: renal corpuscle cells (indicated with arrows) 400X magnifiation.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

EMG1 Antibody Summary

Immunogen
Synthetic peptides corresponding to EMG1 (EMG1 nucleolar protein homolog (S. cerevisiae)). The peptide sequence was selected from the N terminal of EMG1. Peptide sequence SLETVKVGKTYELLNCDKHKSILLKNGRDPGEARPDITHQSLLMLMDSPL.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
EMG1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100 - 1:2000
  • Immunohistochemistry 1:10 - 1:500
  • Immunohistochemistry-Paraffin 1:10 - 1:500
Application Notes
This is a rabbit polyclonal antibody against EMG1 and was validated on Western Blot and Immunohistochemistry-Paraffin.
Reviewed Applications
Read 1 Review rated 1
using
NBP1-57410 in the following applications:

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS, 2% Sucrose.
Preservative
0.09% Sodium Azide
Purity
Immunogen affinity purified

Notes

The addition of 50% glycerol is optional for those storing this antibody at -20C and not aliquoting smaller units. However, please note that glycerol may interrupt some downstream antibody applications and should be added with caution.

Alternate Names for EMG1 Antibody

  • 18S rRNA (pseudouridine-N1-)-methyltransferase NEP1
  • BWCNS
  • C2F18S rRNA Psi1248 methyltransferase
  • EMG1 nucleolar protein homolog (S. cerevisiae)
  • essential for mitotic growth 1
  • FLJ60792
  • Grcc2f
  • NEP1EC 2.1.1.-
  • Nucleolar protein EMG1 homolog
  • probable ribosome biogenesis protein NEP1
  • Protein C2f
  • Ribosome biogenesis protein NEP1

Background

EMG1 involved in 40S ribosomal subunit biogenesis. It seems to play a role a methylation reaction in pre-rRNA processing.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for EMG1 Antibody (NBP1-57410) (0)

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Review for EMG1 Antibody (NBP1-57410) (1) 11

Average Rating: 1
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NBP1-57410:
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WB
(1)
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Images Ratings Applications Species Date Details
Western Blot EMG1 NBP1-57410
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reviewed by:
Anonymous
WB Human 03/25/2019
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Summary

ApplicationWestern Blot
Sample Testedb-cell lymphocyte,B-cell whole cell lysate 40ug
SpeciesHuman
Lotqc10242

Comments

CommentsI used this antibody at a 1:500 dilution (1ug/mL) overnight 4C. I ran 40ug of total cell lysate from B-cell lymphocytes. I also ran the same sample with a second EMG1 antibody (Origene TA501209) which had a very strong signal. The image submitted is a 3 minute exposure. A longer exposure did not reveal any specific bands.

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Recent Reviews

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1

Anonymous
03/25/2019
Application: WB
Species: Human

Bioinformatics

Gene Symbol EMG1
Uniprot