Synthetic peptides corresponding to EMG1 (EMG1 nucleolar protein homolog (S. cerevisiae)). The peptide sequence was selected from the N terminal of EMG1. Peptide sequence SLETVKVGKTYELLNCDKHKSILLKNGRDPGEARPDITHQSLLMLMDSPL.
Immunogen affinity purified
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The addition of 50% glycerol is optional for those storing this antibody at -20C and not aliquoting smaller units. However, please note that glycerol may interrupt some downstream antibody applications and should be added with caution.
I used this antibody at a 1:500 dilution (1ug/mL) overnight 4C. I ran 40ug of total cell lysate from B-cell lymphocytes. I also ran the same sample with a second EMG1 antibody (Origene TA501209) which had a very strong signal. The image submitted is a 3 minute exposure. A longer exposure did not reveal any specific bands.
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