EMG1 Antibody (1B8)

Images

 
Western Blot: EMG1 Antibody (1B8) [NBP2-02575] Analysis of extracts (35ug) from 9 different cell lines by using anti-EMG1 monoclonal antibody.
Immunocytochemistry/ Immunofluorescence: EMG1 Antibody (1B8) [NBP2-02575] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY EMG1.
Flow Cytometry: EMG1 Antibody (1B8) [NBP2-02575] - Analysis of Jurkat cells, using anti-EMG1 antibody, (Red), compared to a nonspecific negative control antibody (Blue).
Western Blot: EMG1 Antibody (1B8) [NBP2-02575] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY EMG1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ...read more
Flow Cytometry: EMG1 Antibody (1B8) [NBP2-02575] - Analysis of Hela cells, using anti-EMG1 antibody, (Red), compared to a nonspecific negative control antibody (Blue).

Product Details

Summary
Reactivity Hu, Ca, PmSpecies Glossary
Applications WB, Flow, ICC/IF
Clone
1B8
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

EMG1 Antibody (1B8) Summary

Immunogen
Full length human recombinant protein of human EMG1(NP_006322) produced in HEK293T cell.
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
EMG1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000-2000
  • Flow Cytometry 1:100
  • Immunocytochemistry/Immunofluorescence 1:100

Reactivity Notes

Human, Dog, Monkey

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for EMG1 Antibody (1B8)

  • 18S rRNA (pseudouridine-N1-)-methyltransferase NEP1
  • BWCNS
  • C2F18S rRNA Psi1248 methyltransferase
  • EMG1 nucleolar protein homolog (S. cerevisiae)
  • essential for mitotic growth 1
  • FLJ60792
  • Grcc2f
  • NEP1EC 2.1.1.-
  • Nucleolar protein EMG1 homolog
  • probable ribosome biogenesis protein NEP1
  • Protein C2f
  • Ribosome biogenesis protein NEP1

Background

This gene encodes an essential, conserved eukaryotic protein involved in ribosome biogenesis. In yeast, the related protein is a component of the small subunit processome and is essential for biogenesis of the ribosomal 40S subunit. A mutation in this gene has been associated with Bowen-Conradi syndrome. COMPLETENESS: complete on the 3' end.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol EMG1
Entrez