ELSPBP1 Antibody - Azide and BSA Free

Western Blot: ELSPBP1 Antibody [NBP3-04400] - Analysis of extracts of various cell lines, using ELSPBP1 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Bas

Immunohistochemistry: ELSPBP1 Antibody [NBP3-04400] - Analysis of rat testis using ELSPBP1 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunocytochemistry/ Immunofluorescence: ELSPBP1 Antibody - Azide and BSA Free [NBP3-04400] - Immunofluorescence analysis of paraffin-embedded mouse testis using ELSPBP1 Rabbit pAb (A15882) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

• Reactivity: Mu, Rt
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free

ELSPBP1 Antibody - Azide and BSA Free Summary

• Description: Novus Biologicals Rabbit ELSPBP1 Antibody - Azide and BSA Free (NBP3-04400) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 110-223 of human ELSPBP1 (NP_071425.3). QQWKFCETNEYGGNSLRKPCIFPSIYRNNVVSDCMEDESNKLWCPTTENMDKDGKWSFCADTRISALVPGFPCHFPFNYKNKNYFNCTNEGSKENLVWCATSYNYDQDHTWVYC
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ELSPBP1
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:50 - 1:200
  • Immunohistochemistry
  • Western Blot 1:500 - 1:2000
• Theoretical MW: 26 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 50% glycerol
• Preservative: 0.01% Thimerosal
• Purity: Affinity purified

Alternate Names for ELSPBP1 Antibody - Azide and BSA Free

• E12epididymal protein 12
• EDDM12
• Epididymal secretory protein 12
• epididymal sperm binding protein 1
• epididymal sperm-binding protein 1
• HE12

Background

The protein encoded by the ELSPBP1 gene belongs to the sperm-coating protein family of epididymal origin. This protein and itscanine homolog are the first known examples of proteins with four tandemly arranged fibronectin type 2 (Fn2) domainsin the Fn2-module protein family. (provided by RefSeq)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including Methotrexate, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information, go to www.P65Warnings.ca.gov

Bioinformatics

• Gene Symbol: ELSPBP1