eIF5A Antibody (2E9)

Images

 
Western Blot: eIF5A Antibody (AT2E9) [NBP1-48352] - PAGE, transferred to NC membrane and probed with anti-human EIF5A (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ...read more
Immunocytochemistry/ Immunofluorescence: eIF5A Antibody (AT2E9) [NBP1-48352] - Human HeLa cells stained with Hoechst 33342 (Blue) for chromatin staining and monoclonal anti-human EIF5A antibody (1:500) with Texas Red ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, ICC/IF
Clone
2E9
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

eIF5A Antibody (2E9) Summary

Immunogen
Recombinant human EIF5A (1-154aa) purified from E. coli.
Specificity
Human EIF5A (1-154aa)
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
EIF5A
Purity
Protein G purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 1:250
  • ELISA 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence 1:250-1:500
Application Notes
The antibody has been tested by ELISA, Western blot and Immunofluorescence analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot analysis and Immunofluorescence is 1:250-500. Recommended starting dilution is 1:250

Reactivity Notes

Human.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4) and 10% Glycerol
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Protein G purified

Alternate Names for eIF5A Antibody (2E9)

  • eIF4D
  • eIF-4D
  • eIF5A
  • EIF-5A
  • EIF5A1
  • eIF-5A1
  • eIF-5A-1
  • eIF5AI
  • Eukaryotic initiation factor 5A isoform 1
  • eukaryotic initiation factor 5A
  • eukaryotic translation initiation factor 5A
  • eukaryotic translation initiation factor 5A-1
  • MGC104255
  • MGC99547
  • Rev-binding Factor

Background

EIF5A, previously known as EIF4D and IF-M2Ba, is a highly conserved 17-kDa protein that was originally characterized as a translation initiation factor, promoting the formation of the first peptide bond in mRNA translation in vitro. This is best positioned as a factor that controls the balance between cellular proliferation and death, depending upon the nature of cellular stress. Moreover, mutational analysis using human EIF5A to complement an EIF5A null strain of S. cerevisiae supports a role for EIF5A in protein synthesis. The activity of EIF5A is modulated by the covalent addition of the spermidine derivative hypusine to lysine residue 50.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NBP1-48352AF405
Alexa Fluor 488 Labeled NBP1-48352AF488
Alexa Fluor 647 Labeled NBP1-48352AF647
Alexa Fluor 700 Labeled NBP1-48352AF700
DyLight 350 Labeled NBP1-48352UV
DyLight 405 Labeled NBP1-48352V
DyLight 488 Labeled NBP1-48352G
DyLight 680 Labeled NBP1-48352FR
DyLight 755 Labeled NBP1-48352IR

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Bioinformatics

Gene Symbol EIF5A
Entrez
Uniprot