eIF4E [p Ser209] Antibody

Images

 
Western Blot: eIF4E [p Ser209] Antibody [NBP1-51445] - eIF4E (S209) pAb in extracts from 293 Anisomycin cells.
Immunohistochemistry-Paraffin: eIF4E [p Ser209] Antibody [NBP1-51445] Human brain tissue.

Product Details

Summary
Product Discontinued
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Order Details


    • Catalog Number
      NBP1-51445
    • Availability
      Product Discontinued

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eIF4E [p Ser209] Antibody Summary

Immunogen
Synthetic phosphopeptide derived from human eIF4E around the phosphorylation site of Serine 209.
Modification
p Ser209
Specificity
p-eIF4E (S209) pAb detects endogenous levels of eIF4E protein only when phosphorylated at S209.
Clonality
Polyclonal
Host
Rabbit
Gene
EIF4E
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:1000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:50-1:200
Application Notes
Western blot (WB) analyzes of p-eIF4E (S209) pAb in extracts from 293 Anisomycin cells.
Immunohistochemistry (IHC) analyzes of p-eIF4E (S209) pAb in paraffin-embedded human brain tissue.
Theoretical MW
25 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Cross reacts with Human, Mouse and Rat.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2)
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for eIF4E [p Ser209] Antibody

  • CBP
  • eIF4E
  • eIF-4E
  • EIF4E1
  • EIF4EL1
  • EIF4EL1MGC111573
  • eIF-4F 25 kDa subunit
  • EIF4F
  • eukaryotic translation initiation factor 4E
  • eukaryotic translation initiation factor 4E-like 1
  • mRNA cap-binding protein

Background

The initiation of protein synthesis in eukaryotic cells is regulated by interactions between protein initiation factors and RNA molecules. The eukaryotic initiation complex eIF4F exists in vitro as a trimeric complex of eIF4G, eIF4E, and eIF4A. Together, the complex allows ribosome binding to mRNA by inducing the unwinding of mRNA secondary structures. eIF4E is a 25 kDa protein that binds to the mRNA "cap" during an early step in the initiation of protein synthesis. eIF4A is a 46 kDa protein that acts as an ATP-dependent RNA helicase. eIF4G is 154 kDa protein that acts as a bridge between eIF4E, eIF4A, and the eIF3 complex.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol EIF4E
Entrez