EIF3J Antibody

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Western Blot: EIF3J Antibody [NBP2-16301] - Sample (30 ug of whole cell lysate) A: Raji 12% SDS PAGE gel, diluted at 1:1000.

Product Details

Summary
Reactivity Hu, Rt, Bv, Ch, RMSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.91 mg/ml

Order Details

EIF3J Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human EIF3J. The exact sequence is proprietary.
Localization
Cytoplasm
Predicted Species
Rat (97%), Chicken (94%), Bovine (97%), Rhesus Macaque (99%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
EIF3J
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
Theoretical MW
29 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7.0), 0.1M Glycine and 20% Glycerol
Preservative
0.01% Thimerosal
Concentration
0.91 mg/ml
Purity
Immunogen affinity purified

Alternate Names for EIF3J Antibody

  • eIF3 p35
  • eIF3-alpha
  • eIF3j
  • eIF3-p35
  • EIF3S1eIF-3-alpha
  • Eukaryotic translation initiation factor 3 subunit 1
  • eukaryotic translation initiation factor 3 subunit J
  • eukaryotic translation initiation factor 3, subunit 1 (alpha, 35kD)
  • eukaryotic translation initiation factor 3, subunit 1 alpha, 35kDa
  • eukaryotic translation initiation factor 3, subunit J

Background

Eukaryotic initiation factor-3 (EIF3) has a molecular mass of about 600 kD and contains 13 nonidentical protein subunits, including EIF3J. EIF3 plays a central role in binding of initiator methionyl-tRNA and mRNA to the 40S ribosomal subunit to form the 40S initiation complex (Fraser et al., 2004 [PubMed 14688252]; Fraser et al., 2007 [PubMed 17588516]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol EIF3J
Uniprot