eIF2A Antibody

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Western Blot: eIF2A Antibody [NB100-78297] - Hela cell extract was resolved by electrophoresis, transferred to nitrocellulose, and probed with rabbit anti-eIF-2a antibody. Proteins were visualized using a donkey ...read more

Product Details

Summary
Product Discontinued
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Order Details


    • Catalog Number
      NB100-78297
    • Availability
      Product Discontinued

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eIF2A Antibody Summary

Immunogen
Recombinant (partial), C-terminal
Specificity
eIF-2 alpha
Clonality
Polyclonal
Host
Rabbit
Gene
EIF2A
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
Application Notes
Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 10 mul per 5 ml antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application.
Positive Control
eIF2A Lysate (NBL1-10174)

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 50% Glycerol
Preservative
0.09% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for eIF2A Antibody

  • EIF-2
  • EIF-2A
  • EIF2AEIF2
  • EIF-2alpha
  • eukaryotic translation initiation factor 2, subunit 1 alpha, 35kDa
  • subunit 1 (alpha, 35kD )

Background

eIF-2 alpha (also known as eukaryotic translation initiation factor 2, subunit 1 alpha) is a 35 kD member of the eukaryotic translation initiation factor 2 family. This cytoplasmic protein is ubiquitously expressed and can downregulate protein synthesis when phosphorylated by PKR kinase under stress conditions (hypoxia, viral infection, ER stress). Increased expression of eIF-2 alpha has been linked to progression of thyroid carcinoma.Signaling inhibited by p58 (IPK) and PKR phosphorylation. Can attenuate the unfolded protein response and inhibits translation. This protein has been shown to interact with eIF2B alpha, eIF2B delta, eIF2B beta subunit 2, p67, p68, and PKR. eIF-2 alpha is a target for cleavage by caspase 3, caspase 6, caspase 8, and caspase 10. The Poly6067 antibody recognizes both mouse and human eIF-2 alpha and has been shown to be useful for Western blotting.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Additional eIF2A Products

Bioinformatics Tool for eIF2A Antibody (NB100-78297)

Discover related pathways, diseases and genes to eIF2A Antibody (NB100-78297). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Diseases for eIF2A Antibody (NB100-78297)

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Research Areas for eIF2A Antibody (NB100-78297)

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Blogs on eIF2A.

eIF2alpha - a regulator of global translation in response to cellular stress
Eukaryotic initiation factor 2 (eIF2) regulates global protein translation by binding to Met-tRNA and the 40S ribosome to form the pre-initiation complex. eIF2 is a heterotrimer consisting of alpha, beta, and gamma subunits. The 36kDA eIF2a subuni...  Read full blog post.

PKR - Mediating cellular stress responses through multiple signaling pathways
Protein kinase R (PKR) is an intracellular stress-sensing protein that is able to detect and respond to viral infections. While PKR is able to sense and respond to a variety of signals, dsRNA is a well-characterized ligand. dsRNA produced during v...  Read full blog post.

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Bioinformatics

Gene Symbol EIF2A
Entrez