eIF2 alpha/EIF2S1 Antibody (3H4)

Images

 
Western Blot: EIF2S1 Antibody (3H4) [NBP2-02669] Analysis of extracts (35ug) from 9 different cell lines by using anti-eIF2A monoclonal antibody.
Immunocytochemistry/ Immunofluorescence: EIF2S1 Antibody (3H4) [NBP2-02669] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY eIF2A.
Immunohistochemistry-Paraffin: EIF2S1 Antibody (3H4) [NBP2-02669] - Staining of paraffin-embedded Human prostate tissue using anti-eIF2A mouse monoclonal antibody.
Flow Cytometry: EIF2S1 Antibody (3H4) [NBP2-02669] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-eIF2A antibody, and then analyzed ...read more
Western Blot: EIF2S1 Antibody (3H4) [NBP2-02669] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY eIF2A (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates ...read more
Immunohistochemistry-Paraffin: EIF2S1 Antibody (3H4) [NBP2-02669] - Staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-eIF2A mouse monoclonal antibody.
Immunohistochemistry-Paraffin: EIF2S1 Antibody (3H4) [NBP2-02669] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-eIF2A mouse monoclonal antibody.
Immunohistochemistry-Paraffin: EIF2S1 Antibody (3H4) [NBP2-02669] - Staining of paraffin-embedded Human Kidney tissue using anti-eIF2A mouse monoclonal antibody.
Immunohistochemistry-Paraffin: EIF2S1 Antibody (3H4) [NBP2-02669] - Staining of paraffin-embedded Human pancreas tissue using anti-eIF2A mouse monoclonal antibody.

Product Details

Summary
Reactivity Hu, Rt, Ca, PmSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IHC-P
Clone
3H4
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

eIF2 alpha/EIF2S1 Antibody (3H4) Summary

Immunogen
Full length human recombinant protein of human EIF2S1 (NP_004085) produced in HEK293T cell.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
EIF2S1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000-2000
  • Flow Cytometry 1:100
  • Immunocytochemistry/Immunofluorescence 1:100
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:50

Reactivity Notes

Human, Dog, Rat, Monkey

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for eIF2 alpha/EIF2S1 Antibody (3H4)

  • eIF2 alpha
  • EIF2
  • EIF-2
  • EIF2A
  • EIF-2A
  • EIF-2alpha
  • EIF2S1 eukaryotic translation initiation factor 2, subunit 1 alpha, 35kDa
  • EIF2S1

Background

EIF2S1, also known as eIF2a, is the alpha subunit of the translation initiation factor eIF2 complex which catalyzes the first regulated step of protein synthesis initiation, promoting the binding of the initiator tRNA to 40S ribosomal subunits. The phosphorylation state of eIF2S1 controls the rate of tRNA translation. When eIF2a is not phosphorylated, translation occurs at a normal rate. However, upon phosphorylation by one of several kinases, eIF2S1 is stabilized, thus preventing the GDP/GTP exchange reaction and slowing translation. Recombinant human EIF2S1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP1-77266
Species: Hu, Mu, Rt
Applications: WB, ELISA, ICC/IF, IHC, IHC-P, IF
AF8519
Species: Hu, Mu, Rt
Applications: WB
NBP1-83210
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NBP2-16293
Species: Hu
Applications: WB, ICC/IF
NBP1-32783
Species: Hu, Mu
Applications: WB, ICC/IF
NBP2-01370
Species: Hu, Ca
Applications: WB, ICC/IF, IHC, IHC-P
AF6260
Species: Hu
Applications: WB
NBP1-52556
Species: Hu, Mu, Po, Bt, Bv, Ca, Mk, Pm, Rb
Applications: IHC-P
NBP1-85727
Species: Hu
Applications: ICC/IF, IHC, IHC-P
NB600-1335
Species: Hu, Mu, Rt, Pm
Applications: WB, Simple Western, GS, ICC/IF, IHC, IHC-P, IP
NB100-81898
Species: Hu, Po
Applications: WB, ICC/IF, IHC, IHC-P
NBP2-21577
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P, IP
H00056731-M02
Species: Hu
Applications: WB, ELISA, ICC/IF
NB100-93308
Species: Hu
Applications: WB, IP
NBP2-24632
Species: Hu, Mu, Rt, Ca, Pm
Applications: WB, IHC-P
NBP2-01787
Species: Hu, Mu, Rt, Ca, Mk
Applications: WB, Flow, ICC/IF, IHC, IHC-P
NBP1-78017
Species: Hu, Mu
Applications: WB, ELISA, ICC/IF, IHC, IHC-P, IP
NBP1-86184
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P, IF
NBP2-38126
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NBP2-37620
Species: Hu
Applications: WB, ELISA, ICC/IF, IHC

Publications for eIF2 alpha/EIF2S1 Antibody (NBP2-02669) (0)

There are no publications for eIF2 alpha/EIF2S1 Antibody (NBP2-02669).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for eIF2 alpha/EIF2S1 Antibody (NBP2-02669) (0)

There are no reviews for eIF2 alpha/EIF2S1 Antibody (NBP2-02669). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for eIF2 alpha/EIF2S1 Antibody (NBP2-02669) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional eIF2 alpha/EIF2S1 Products

Bioinformatics Tool for eIF2 alpha/EIF2S1 Antibody (NBP2-02669)

Discover related pathways, diseases and genes to eIF2 alpha/EIF2S1 Antibody (NBP2-02669). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for eIF2 alpha/EIF2S1 Antibody (NBP2-02669)

Discover more about diseases related to eIF2 alpha/EIF2S1 Antibody (NBP2-02669).
 

Pathways for eIF2 alpha/EIF2S1 Antibody (NBP2-02669)

View related products by pathway.

PTMs for eIF2 alpha/EIF2S1 Antibody (NBP2-02669)

Learn more about PTMs related to eIF2 alpha/EIF2S1 Antibody (NBP2-02669).

Blogs on eIF2 alpha/EIF2S1

There are no specific blogs for eIF2 alpha/EIF2S1, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our eIF2 alpha/EIF2S1 Antibody (3H4) and receive a gift card or discount.

Bioinformatics

Gene Symbol EIF2S1
Entrez