eIF2 alpha/EIF2S1 Antibody (1B10)

Images

 
Western Blot: EIF2S1 Antibody (1B10) [NBP2-02725] Analysis of extracts (35ug) from 9 different cell lines by using anti-eIF2A monoclonal antibody.
Immunohistochemistry-Paraffin: EIF2S1 Antibody (1B10) [NBP2-02725] - Staining of paraffin-embedded Human liver tissue using anti-eIF2A mouse monoclonal antibody.
Western Blot: EIF2S1 Antibody (1B10) [NBP2-02725] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY eIF2A (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates ...read more
Immunohistochemistry-Paraffin: EIF2S1 Antibody (1B10) [NBP2-02725] - Staining of paraffin-embedded Human colon tissue using anti-eIF2A mouse monoclonal antibody.

Product Details

Summary
Product Discontinued
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Order Details


    • Catalog Number
      NBP2-02725
    • Availability
      Product Discontinued

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eIF2 alpha/EIF2S1 Antibody (1B10) Summary

Immunogen
Full length human recombinant protein of human EIF2S1 (NP_004085) produced in HEK293T cell.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
EIF2S1
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 1:1000-2000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:50

Reactivity Notes

Human, Dog, Rat, Monkey

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for eIF2 alpha/EIF2S1 Antibody (1B10)

  • eIF2 alpha
  • EIF2
  • EIF-2
  • EIF2A
  • EIF-2A
  • EIF-2alpha
  • EIF2S1 eukaryotic translation initiation factor 2, subunit 1 alpha, 35kDa
  • EIF2S1

Background

EIF2S1, also known as eIF2a, is the alpha subunit of the translation initiation factor eIF2 complex which catalyzes the first regulated step of protein synthesis initiation, promoting the binding of the initiator tRNA to 40S ribosomal subunits. The phosphorylation state of eIF2S1 controls the rate of tRNA translation. When eIF2a is not phosphorylated, translation occurs at a normal rate. However, upon phosphorylation by one of several kinases, eIF2S1 is stabilized, thus preventing the GDP/GTP exchange reaction and slowing translation. Recombinant human EIF2S1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol EIF2S1
Entrez