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EGR1 Antibody

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Product Details

Summary
Product Discontinued
View other related EGR1 Primary Antibodies

Order Details


    • Catalog Number
      NB600-873
    • Availability
      Product Discontinued

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EGR1 Antibody Summary

Immunogen
Synthetic peptide: GEQPYEHLTAESFPDI-C conjugated to KLH, corresponding to amino acids 93-108 of Human Egr1.
Localization
Nuclear
Specificity
We have tested this by Western blotting on Hela Nuclear lysate, we detected 2 bands at just over 50kD, but no band at 83kD (expected height based on data from other antibodies).
Clonality
Polyclonal
Host
Rabbit
Gene
EGR1
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • ELISA 1:4000 - 1:16000
  • Immunohistochemistry 2 ug/ml - 20 ug/ml
  • Western Blot 1:500 - 1:3000
Application Notes
This affinity purified antibody has been tested for use in ELISA, immunohistochemistry and western blot. Expect a band at ~58 kDa in size corresponding - EGR-1 by western blotting in the appropriate cell lysate or extract.
Control
EGR1 Overexpression Lysate

Reactivity Notes

Expected to cross-react with Human (100% identity with immunogen), Mouse (86% identity with immunogen), Rat (86% identity with immunogen) due to sequence homology. Not yet tested in other species.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for EGR1 Antibody

  • AT225Transcription factor ETR103
  • early growth response 1
  • early growth response protein 1
  • EGR1
  • EGR-1
  • G0S30
  • KROX24
  • KROX-24
  • Nerve growth factor-induced protein A
  • NGFIA
  • NGFI-ATranscription factor Zif268
  • TIS8
  • ZIF-268
  • Zinc finger protein Krox-24
  • ZNF225
  • ZNF225Zinc finger protein 225

Background

This protein belongs to the EGR family of C2H2-type zinc-finger proteins. It is a nuclear protein and functions as a transcriptional regulator. The products of target genes it activates are required for differentitation and mitogenesis. Studies suggest this is a cancer suppresor gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NB600-873
EGR1 Antibody
Species: Hu, Mu, Rt
Applications: WB, ELISA, IHC

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Product General Protocols

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Video Protocols

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Control Lysate(s)

EGR1 Overexpression Lysate

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol EGR1
Entrez