EGLN2/PHD1 Antibody [FITC]

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Product Details

Summary
Product Discontinued
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Order Details


    • Catalog Number
      NB100-310F
    • Availability
      Product Discontinued

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EGLN2/PHD1 Antibody [FITC] Summary

Immunogen
The epitope maps to a region between residue 350 and the C-terminus (residue 407) of human PHD1/HIF Prolyl Hydroxylase 1 using the numbering given in entry NP_444274.1 (GeneID 112398).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
EGLN2
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
Application Notes
This PHD1/HIF Prolyl Hydroxylase 1 antibody is useful for Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Immunohistochemistry and Western Blot. In WB a band is seen at 45-50 kDa. In ICC/IF, cytoplasmic and nuclear staining was observed in HeLa cells. IP (PMID: 21282188). IHC (PMID 21877141). Use in Immunohistochemistry-Paraffin and Electron Microscopy reported in scientific literature (PMID 17003483)

The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
48 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Rat reactivity reported in scientific literature (PMID: 17003483).

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
PBS
Preservative
0.05% Sodium Azide
Purity
Immunogen affinity purified

Notes

This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for EGLN2/PHD1 Antibody [FITC]

  • DKFZp434E026
  • EC 1.14.11
  • EC 1.14.11.-
  • egl nine homolog 2 (C. elegans)
  • egl nine homolog 2
  • EGLN2
  • EIT6
  • Estrogen-induced tag 6
  • FLJ95603
  • HIFPH1 EGL nine (C.elegans) homolog 2
  • HIFPH1
  • HIF-PH1
  • HIF-prolyl hydroxylase 1
  • HPH-1
  • HPH-3
  • Hypoxia-inducible factor prolyl hydroxylase 1
  • PHD1
  • Prolyl hydroxylase domain-containing protein 1

Background

HIF prolyl 4-hydroxylases (PHD) are proyl hydroxylase domain-containing enzymes (PHD1/ Egln2, PHD2/ Egln1, PHD3/ Egln3, and P4H-TM) that facilitate physiological reactions to hypoxic stress by modulating HIF1alpha expression levels. HIF-alpha is regulated by hydroxylation, both by a family of PHDs leading to ubiquitination and proteasomal degradation, and by transcriptional inactivation following asparaginyl hydroxylation by FIH (factor inhibiting HIF). HIF Prolyl Hydroxylase 1 (HIF-PH1/EGLN2/PHD1) is localized in the nucleus and interacts with SIAH2, LIMD1, WTIP and AJUBA. In normal oxygen conditions, HIF-PH1 catalyzes the post-translational formation of 4-hydroxyproline in HIF- alpha and hydroxylates a specific prolines found in NODD/CODD of HIF1A, and also hydroxylates HIF2A with preference for CODD site for HIF1A/HIF2A. VHL (von Hippel-Lindau ubiquitination) complex then targets hydroxylated HIFs to facilitate their proteasomal degradation. Under hypoxic stress conditions, the hydroxylation reaction is attenuated allowing HIFs to escape degradation resulting in their translocation to the nucleus, heterodimerization with HIF1B, and increased expression of hypoxy-inducible genes. HIF-PH1 is involved in regulating hypoxia tolerance and apoptosis in cardiac and skeletal muscle, tumor angiogenesis and susceptibility to normoxic oxidative neuronal death.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

ICC/IF Video Protocol

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Bioinformatics

Gene Symbol EGLN2
Entrez