EGFR Antibody (6E3)

Images

 
Western Blot: EGF R/ErbB1 Antibody (6E3) [NBP1-74038] - The extracts of HaCaT (35ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human EGFR (1:500 or 1000). Proteins were visualized ...read more
Immunocytochemistry/ Immunofluorescence: EGF R/ErbB1 Antibody (6E3) [NBP1-74038] - Immunofluorescence staining analysis of EGFR protein in HeLa cells with mouse monoclonal anti-humanEGFR antibody clone 6E3 at 1:100 ...read more
Flow Cytometry: EGF R/ErbB1 Antibody (6E3) [NBP1-74038] - Flow cytometry analysis of EGFR protein in HeLa cells using EGFR/ErbB1 antibody (clone 6E3) at a concentration of 2-5ug for 1x10^6 cells. The secondary antibody ...read more

Product Details

Summary
Product Discontinued
View other related EGFR Primary Antibodies

Order Details


    • Catalog Number
      NBP1-74038
    • Availability
      Product Discontinued

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EGFR Antibody (6E3) Summary

Immunogen
Recombinant human EGFR (424-605aa) purified from E. coli
Specificity
Anti-human EGFR mAb, clone 6E3, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human EGFR protein.
Isotype
IgG2b Kappa
Clonality
Monoclonal
Host
Mouse
Gene
EGFR
Purity
Protein G purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:1000
  • ELISA
  • Flow Cytometry 2-5ug/1X10^6 cells
  • Immunocytochemistry/Immunofluorescence 1:100

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4) and 10% Glycerol
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Protein G purified

Alternate Names for EGFR Antibody (6E3)

  • avian erythroblastic leukemia viral (v-erb-b) oncogene homolog
  • cell growth inhibiting protein 40
  • cell proliferation-inducing protein 61
  • EC 2.7.10
  • EC 2.7.10.1
  • EGF R
  • EGFR
  • epidermal growth factor receptor (avian erythroblastic leukemia viral (v-erb-b)oncogene homolog)
  • epidermal growth factor receptor
  • ErbB
  • ErbB1
  • ERBB1PIG61
  • HER1
  • HER-1
  • mENA
  • Proto-oncogene c-ErbB-1
  • Receptor tyrosine-protein kinase erbB-1

Background

Epidermal growth factor receptor (EGFR) is a 170 kDa membrane protein first identified as a binding partner of EGF. Also, EGFR phosphorylated itself (autophosphorylation) in response to EGF treatment. The overexpression of EGFR in various epithelial tumors was widely reported and it has been substantiated that deregulation of EGFR itself and its signaling pathways an important role in human cancers.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for EGFR Antibody (NBP1-74038) (0)

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Secondary Antibodies

 

Isotype Controls

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Mitogen-activated protein kinases (MAPKs) are important signaling proteins needed to transmit and relay extracellular stimuli and to illicit intracellular responses (1). The MAPK family of proteins are serine/threonine kinases that are able to phos...  Read full blog post.

Using EGF Protein from Novus Biologicals
EGF (epidermal growth factor) stimulates differentiation, proliferation and cell growth by binding to its receptor, EGFR. EGF was first discovered in the mouse submandibular gland in 1986 by Stanley Cohen of Vanderbilt University, leading to a Nobel P...  Read full blog post.

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Bioinformatics

Gene Symbol EGFR
Entrez
Uniprot