EGF R/ErbB1 [p Tyr1197] Antibody


Western Blot: EGF R/ErbB1 [p Tyr1197] Antibody [NBP1-77936] - Lane 1: unstimulated A431 cells. Lane 2: A431 cells stimulated with EGF (50 ng/ml for 15 min). Load: 30 ug per lane. Primary antibody: EGFR pY1197 antibody more
Immunohistochemistry-Paraffin: EGF R/ErbB1 [p Tyr1197] Antibody [NBP1-77936] - Tissue: placental trophoblasts. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: EGFR pY1197 more

Product Details

Reactivity HuSpecies Glossary
Applications WB, ELISA, IHC, IHC-P
Please see the vial label for concentration. If unlisted please contact technical services.

Order Details

EGF R/ErbB1 [p Tyr1197] Antibody Summary

This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 1189-1199 of human EGFR protein.
p Tyr1197
This antibody is directed against the phosphorylated form of human EGFR protein at the pY1197 residue.
Immunogen affinity purified
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Packaging, Storage & Formulations

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 Stabilizer: None
0.01% Sodium Azide
Immunogen affinity purified


  • Western Blot 1:250-1:1500
  • ELISA 1:4000-1:20000
  • Immunohistochemistry 5 ug/ml
  • Immunohistochemistry-Paraffin 1:10-1:500
Application Notes
Expect a band approximately 170 kDa in size corresponding - phosphorylated EGFR protein by western blotting in the appropriate cell lysate or extract. Less than 5.0% reactivity is observed against the non-phosphorylated form of the immunizing peptide. This antibody is phospho specific for pY1197 of EGFR protein.

Reactivity Notes

Reactive in human

Alternate Names for EGF R/ErbB1 [p Tyr1197] Antibody

  • avian erythroblastic leukemia viral (v-erb-b) oncogene homolog
  • cell growth inhibiting protein 40
  • cell proliferation-inducing protein 61
  • EC 2.7.10
  • EC
  • EGFR
  • epidermal growth factor receptor (avian erythroblastic leukemia viral (v-erb-b)oncogene homolog)
  • epidermal growth factor receptor
  • ERBB
  • ErbB1
  • ERBB1PIG61
  • HER1
  • HER-1
  • mENA
  • Proto-oncogene c-ErbB-1
  • Receptor tyrosine-protein kinase erbB-1


EGFR is a transmembrane glycoprotein that is a member of a family of protein tyrosine kinases crucial in maintaining a normal balance in cell growth and development. Growth factor receptors are involved not only in promoting the proliferation of normal cells but also in the aberrant growth of many types of human tumors. For example, the epidermal growth factor receptor (EGFR) is mutated and/or overexpressed in many common solid human squamous cell carcinomas including breast, brain, bladder, lung, gastric, head & neck, esophagus, cervix, vulva, ovary, and endometrium. Over-expression of the EGFR gene occurs in carcinomas with and without gene amplification. EGFR and erbB-2 are particularly important in breast cancer because increased production or activation has been associated with poor prognosis. EGFR belongs to a family of growth factor receptors, which also includes ErbB-2/HER-2, ErbB-3/HER-3 and ErbB-4/HER-4. EGFR can heterodimerize with each of the members of this family.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Gene Symbol EGFR

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