Western Blot: EGF R/ErbB1 Antibody [NB100-596] - Whole cell lysate (50 ug) from 293T and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody:Affinity purified rabbit anti-EGFR antibody used for WB at 0.04 ...read more
Immunohistochemistry-Paraffin: EGF R/ErbB1 Antibody [NB100-596] - FFPE section of human placenta. Antibody: Affinity purified rabbit anti-EGFR used at a dilution of 1:1,000 (1ug/ml). Detection: DAB.
Western Blot: EGF R/ErbB1 Antibody [NB100-596] - Detection of Human EGFR on HeLa whole cell lysate using NB100-596.
Western Blot: EGF R/ErbB1 Antibody [NB100-596] - Whole cell lysate (5, 15 and 50 ug) from HeLa cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-EGFR antibody used for WB at 0.04 ug/ml. ...read more
Immunohistochemistry-Paraffin: EGF R/ErbB1 Antibody [NB100-596] - Human placenta. Antibody used at a dilution of 1:1,000.
Proximity Ligation Assay: EGF R/ErbB1 Antibody [NB100-596] - The above image shows representative results for PLA using three color fluorescence, including DAPI stained nuclei (blue), phalloidin stained cytoplasmic ...read more
A synthetic peptide which maps to a region between residues 1150 and the C-terminus (residue 1210) of human Epidermal Growth Factor Receptor using the numbering given in Swiss-Prot entry P00533 (GeneID 1956).
Primate (100%), Monkey (100%). Backed by our 100% Guarantee.
Immunogen affinity purified
Test in a species/application not listed above to receive a full credit towards a future purchase.
Reactivity with mouse EGFR is likely owing to high conservation of sequence between this ortholog and human EGFR at the site to which the epitope maps. Based on 100% sequence identity, this antibody is expected to react with Rhesus Monkey, Gorilla, Chimpanzee, White-tufted-ear marmoset, Crab-eating macaque and Northern white-cheeked gibbon.
This anitbody can be used as the primaryantibody in a PLA assay with the following as secondary antibodies: NB200-171
Epidermal Growth factor receptor (EGFR) is the prototype member of the type 1 receptor tyrosine kinases. EGFR is encoded by the cellular oncogene cerbB1. EGFR has an extracellular ligand binding domain, a single transmembrane region, and cytoplasmic domain which is composed of a tyrosine kinase domain and a carboxy terminal domain. The carboxy terminal domain contains at least four tyrosine autophosphorylation sites. EGFR overexpression in tumors indicates poor prognosis. EGFR overexpression is observed in tumors of the head and neck, brain, bladder, stomach, breast, lung, endometrium, cervix, vulva, ovary, esophagus, stomach and in squamous cell carcinoma.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.