EGF R/ErbB1 Antibody


Western Blot: EGF R/ErbB1 Antibody [NB100-595] - Whole cell lysate (50 ug) from HeLa and MCF7 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-EGFR antibody used for WB at 0.02 ug/ml. more
Immunohistochemistry-Paraffin: EGF R/ErbB1 Antibody [NB100-595] - Human placenta. Antibody used at a dilution of 1:1,000.
Western Blot: EGF R/ErbB1 Antibody [NB100-595] - Detection of Human EGFR on HeLa whole cell lysate using NB100-595.

Product Details

Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P, IP
1.0 mg/ml

Order Details

EGF R/ErbB1 Antibody Summary

A synthetic peptide which maps to a region between residues 1100 and 1150 of human Epidermal Growth Factor Receptor using the numbering given in Swiss-Prot entry P00533 (GeneID 1956).
NB100-595 is specific for human EGFR protein.
Predicted Species
Primate (100%), Monkey (100%)
Immunogen affinity purified
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Packaging, Storage & Formulations

Store at 4C. Do not freeze.
Tris-citrate/phosphate buffer, pH 7 to 8
0.09% Sodium Azide
1.0 mg/ml
Immunogen affinity purified


Application Notes
Optimal working dilutions should be determined experimentally by the investigator. Prepare working dilution immediately before use. Suggested starting dilutions are as follows. Western Blot: 1:10,000 - 1:50,000 Histo/Cytochemistry: not tested *In some cases, the antibody may be diluted further than indicated.
Read Publications using NB100-595.

Reactivity Notes

Based on 100% sequence identity, this antibody is predicted to react with Orangutan, Rhesus Monkey, Gorilla, White-tufted-ear marmoset and Crab-eating macaque.

Alternate Names for EGF R/ErbB1 Antibody

  • avian erythroblastic leukemia viral (v-erb-b) oncogene homolog
  • cell growth inhibiting protein 40
  • cell proliferation-inducing protein 61
  • EC 2.7.10
  • EC
  • EGFR
  • epidermal growth factor receptor (avian erythroblastic leukemia viral (v-erb-b)oncogene homolog)
  • epidermal growth factor receptor
  • ErbB
  • ErbB1
  • ERBB1PIG61
  • HER1
  • HER-1
  • mENA
  • Proto-oncogene c-ErbB-1
  • Receptor tyrosine-protein kinase erbB-1


Epidermal Growth factor receptor (EGFR) is the prototype member of the type 1 receptor tyrosine kinases. EGFR is encoded by the cellular oncogene cerbB1. EGFR has an extracellular ligand binding domain, a single transmembrane region, and cytoplasmic domain which is composed of a tyrosine kinase domain and a carboxy terminal domain. The carboxy terminal domain contains at least four tyrosine autophosphorylation sites. EGFR overexpression in tumors indicates poor prognosis. EGFR overexpression is observed in tumors of the head and neck, brain, bladder, stomach, breast, lung, endometrium, cervix, vulva, ovary, esophagus, stomach and in squamous cell carcinoma.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for EGF R/ErbB1 Antibody (NB100-595)(3)

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies


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Contact Information

Product PDFs


Gene Symbol EGFR

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