Recombinant Mouse EGF Protein

Product Discontinued

Recombinant Mouse EGF Protein Summary

• Description: A partial protein corresponding to EGF.
• Preparation Method: E. Coli.
• Details of Functionality: Measured by mouse BALB/c 3T3 cell proliferation assay. The ED50 is typically 20 pg/ml, corresponding to a specific activity of 5X10^7 units/mg.
• Protein/Peptide Type: Partial Recombinant Protein
• Gene: EGF
• Endotoxin Note: Less than 0.01 ng/ug cytokine as determined by the LAL assay.

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunoassay

Reactivity Notes

This protein was made to the mouse sequence.

Packaging, Storage & Formulations

• Storage: Store at -80C. Avoid freeze-thaw cycles.
• Buffer: PBS, 1.0% BSA
• Preservative: 0.09% Sodium Azide

Notes

Purity: Greater than 97%, as determined by SDS-PAGE

Alternate Names for Recombinant Mouse EGF Protein

• beta-urogastrone
• EGF
• epidermal growth factor (beta-urogastrone)
• epidermal growth factor
• hEGF
• HOMG4
• pro-epidermal growth factor
• URG
• Urogastrone

Background

Epidermal growth factor (EGF) is the member of the EGF family that shares a structural motif, the EGF-like domain, and are synthesized as type I transmembrane precursor proteins that contain several EGF domains in the extracellular region. The mouse EGF precursor has 1217 amino acid residues containing nine EGF domains. The mature protein consists of 53 aa and is generated by proteolytic excision of the EGF domain proximal to the transmembrane region resulting in a molecular mass of 6 kDa. EGF is produced by many cell types and in blood and various body fluids, including milk, urine, saliva, seminal fluid, pancreatic juice, cerebrospinal fluid, and amniotic fluid. EGF can promote proliferation and differentiation of mesenchymal and epithelial cells. EGF is also a mitogen for fibroblasts, epithelial and endothelial cells, and promotes colony formation of epiderma.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: EGF