EGF Antibody (9D7F11)

Images

 
Western Blot: EGF Antibody (9D7F11) [NBP1-47463] - Analysis using EGF mAb against EGF-hIgGFc transfected HEK293 cell lysate.
Immunohistochemistry-Paraffin: EGF Antibody (9D7F11) [NBP1-47463] - Analysis of paraffin-embedded human Uterus tissues using anFtEG mouse mAb
Immunohistochemistry: EGF Antibody (9D7F11) [NBP1-47463] - Analysis of paraffin-embedded human cancer of breast(A), lung cancer(B),showing subcellular localization using anti-EGF mAb with DAB staining.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, IHC, IHC-P
Clone
9D7F11
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

EGF Antibody (9D7F11) Summary

Immunogen
Purified recombinant fragment of human EGF expressed in E. Coli.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
EGF
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:2000
  • ELISA 1:10000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:200-1:1000
Theoretical MW
134 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Human

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Ascites
Preservative
0.03% Sodium Azide
Purity
Unpurified

Alternate Names for EGF Antibody (9D7F11)

  • beta-urogastrone
  • EGF
  • epidermal growth factor (beta-urogastrone)
  • epidermal growth factor
  • HOMG4
  • pro-epidermal growth factor
  • URG
  • Urogastrone

Background

Epidermal growth factor has a profound effect on the differentiation of specific cells in vivo and is a potent mitogenic factor for a variety of cultured cells of both ectodermal and mesodermal origin. The EGF precursor is believed to exist as a membrane-bound molecule which is proteolytically cleaved to generate the 53-amino acid peptide hormone that stimulates cells to divide.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for EGF Antibody (NBP1-47463)

Discover related pathways, diseases and genes to EGF Antibody (NBP1-47463). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Diseases for EGF Antibody (NBP1-47463)

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Research Areas for EGF Antibody (NBP1-47463)

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Blogs on EGF.

Using EGF Protein from Novus Biologicals
EGF (epidermal growth factor) stimulates differentiation, proliferation and cell growth by binding to its receptor, EGFR. EGF was first discovered in the mouse submandibular gland in 1986 by Stanley Cohen of Vanderbilt University, leading to a Nobel P...  Read full blog post.

Myosin is More than Just a Heavy Lifter
Myosin is a well-known, hexameric molecular motor that is a key cytoskeletal component. It consists of a pair of myosin heavy chain subunits (MHC), a pair of essential myosin light chain subunits (MLC), and a pair of regulatory light chain subunits (R...  Read full blog post.

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Bioinformatics

Gene Symbol EGF
Entrez