Western Blot: Eg5 Antibody (10C7/Eg5) [NB100-78467] - la cell extract (Lane 1) or NIH3T3 cell extract (Lane 2) was resolved by electrophoresis, transferred to nitrocellulose and probed with monoclonal anti-Eg5 (Clone ...read more
Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 5 mug antibody per 5 ml antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application. Useful in Immunofluorescence and Immunoprecipitation as reported in Literature.
Read Publications using NB100-78467 in the following applications:
Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.
Alternate Names for Eg5 Antibody (10C7/Eg5)
kinesin family member 11
Kinesin-like protein 1
kinesin-like protein KIF11
Kinesin-like spindle protein HKSP
Kinesin-related motor protein Eg5
thyroid receptor interacting protein 5
Thyroid receptor-interacting protein 5
TR-interacting protein 5
Eg5 (also known as kinesin-like protein KIF11, kinesin-related motor protein Eg5, kinesin-like spindle protein HKSP, and thyroid receptor interacting protein 5 (TRIP5)) is a 119 kD kinesin-like protein family, BimC subfamily. This protein is a catalytic kinesin motor with coiled-coil and Smc domains. Eg5 is localized at the centrosomes, spindle microtubules, and intracellular bridge. This motor protein is required for establishing the bipolar spindle. hEg5 is modified by phosphorylation on Thr927 by Cdc2 to allow association with the spindle apparatus. Eg5 has been shown to interact with the thyroid hormone receptor in presence of thyroid hormone and Cdc2. The 10C7/Eg5 monoclonal antibody has been shown to react with human and mouse Eg5 by Western blot.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.