Eg5 Antibody (10C7/Eg5)

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Western Blot: Eg5 Antibody (10C7/Eg5) [NB100-78467] - la cell extract (Lane 1) or NIH3T3 cell extract (Lane 2) was resolved by electrophoresis, transferred to nitrocellulose and probed with monoclonal anti-Eg5 (Clone ...read more

Product Details

Summary
Product Discontinued
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Order Details


    • Catalog Number
      NB100-78467
    • Availability
      Product Discontinued

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Eg5 Antibody (10C7/Eg5) Summary

Immunogen
Amino Acid: 200-500 of human Eg5
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
KIF11
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/ml
  • Immunocytochemistry/Immunofluorescence 1:10-1:2000
  • Immunoprecipitation 1:10-1:500
Application Notes
Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 5 mug antibody per 5 ml antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application. Useful in Immunofluorescence and Immunoprecipitation as reported in Literature.
Positive Control
Eg5 Lysate (NBL1-12284)
Publications
Read Publications using
NB100-78467 in the following applications:

  • IP
    1 publication
  • WB
    1 publication

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
PBS (pH 7.2)
Preservative
0.09% Sodium Azide
Concentration
0.5 mg/ml
Purity
Protein A or G purified

Alternate Names for Eg5 Antibody (10C7/Eg5)

  • Eg5
  • HKSP
  • kinesin family member 11
  • kinesin-like 1
  • Kinesin-like protein 1
  • kinesin-like protein KIF11
  • Kinesin-like spindle protein HKSP
  • Kinesin-related motor protein Eg5
  • KNSL1TRIP-5
  • thyroid receptor interacting protein 5
  • Thyroid receptor-interacting protein 5
  • TR-interacting protein 5
  • TRIP5EG5

Background

Eg5 (also known as kinesin-like protein KIF11, kinesin-related motor protein Eg5, kinesin-like spindle protein HKSP, and thyroid receptor interacting protein 5 (TRIP5)) is a 119 kD kinesin-like protein family, BimC subfamily. This protein is a catalytic kinesin motor with coiled-coil and Smc domains. Eg5 is localized at the centrosomes, spindle microtubules, and intracellular bridge. This motor protein is required for establishing the bipolar spindle. hEg5 is modified by phosphorylation on Thr927 by Cdc2 to allow association with the spindle apparatus. Eg5 has been shown to interact with the thyroid hormone receptor in presence of thyroid hormone and Cdc2. The 10C7/Eg5 monoclonal antibody has been shown to react with human and mouse Eg5 by Western blot.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Eg5 Antibody (NB100-78467)(5)

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Eg5 Antibody (NB100-78467) (0)

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol KIF11
Entrez
Uniprot