EEF2K Antibody

Product Discontinued

EEF2K Antibody Summary

• Immunogen: The antiserum was produced against synthesized peptide derived from human EEF2K. The immunogen region is: Internal.
• Specificity: EEF2K (Ab-366) Antibody detects endogenous levels of total EEF2K protein.
• Clonality: Polyclonal
• Host: Rabbit
• Gene: EEF2K
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:20000
  • Immunohistochemistry 1:50-1:100
  • Immunohistochemistry-Paraffin 1:50-1:100
  • Western Blot 1:500-1:1000

Reactivity Notes

Human,Rat

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (without Mg2+ and Ca2+, pH 7.4), 0.15M NaCl and 50% Glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for EEF2K Antibody

• Calcium/calmodulin-dependent eukaryotic elongation factor 2 kinase
• calcium/calmodulin-dependent eukaryotic elongation factor-2 kinase
• EC 2.7.11
• EC 2.7.11.20
• eEF-2 kinase
• eEF-2Kcalmodulin-dependent protein kinase III
• elongation factor-2 kinase
• eukaroytic elongation factor 2 kinase
• eukaryotic elongation factor 2 kinase
• eukaryotic elongation factor-2 kinase
• HSU93850
• MGC45041

Background

The activity of the purified eukaryotic elongation-factor-2 kinase (eEF2K) is completely dependent on calcium and calmodulin, and autophosphorylation on serine and threonine residues is calcium/calmodulin-dependent. eEF2K is a ubiquitous protein kinase that phosphorylates and inactivates eEF2, and thus can modulate the rate of polypeptide chain elongation during translation. eEF2K is 120 kDa protein that is detected in skeletal muscle extracts and is phosphorylated rapidly by SAPK4, but poorly by p38, p38g, JNK or ERK 2. SAPK4 phosphorylates eEF2K at Ser 359 and Ser 396 in vitro, causing its inactivation. The phosphorylation of eEF2K at Ser 359 is also induced by insulin-like growth factor-1. Ser 359 is in close proximity to Ser 366 and the Ser 366 residue also becomes phosphorylated in response to growth factors. eEF2K is phosphorylated by p70 S6 kinase at Ser 366 and this results in the inactivation of eEF2K, especially at low (micromolar) calcium concentrations.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: EEF2K
• Uniprot:
  • O00418(Human)