EEF2K Antibody

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Western Blot: EEF2K Antibody [NBP1-51329] - Analysis of EEF2K (G358) pAb in extracts from Jurkat cells.

Product Details

Summary
Product Discontinued
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Order Details


    • Catalog Number
      NBP1-51329
    • Availability
      Product Discontinued

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EEF2K Antibody Summary

Immunogen
Synthetic peptide, corresponding to amino acids 321-370 of Human eEF2K.
Specificity
eEF2K (G358) pAb detects endogenous levels of eEF2K protein.
Clonality
Polyclonal
Host
Rabbit
Gene
EEF2K
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:1000
  • Immunohistochemistry 1:50-1:200
  • Immunohistochemistry-Paraffin 1:50-1:200
Application Notes
Western blot (WB) analysis of eEF2K (G358) pAb in extracts from Jurkat cells.
Theoretical MW
105 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (approx. pH 7.2)
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for EEF2K Antibody

  • Calcium/calmodulin-dependent eukaryotic elongation factor 2 kinase
  • calcium/calmodulin-dependent eukaryotic elongation factor-2 kinase
  • EC 2.7.11
  • EC 2.7.11.20
  • eEF-2 kinase
  • eEF-2Kcalmodulin-dependent protein kinase III
  • elongation factor-2 kinase
  • eukaroytic elongation factor 2 kinase
  • eukaryotic elongation factor 2 kinase
  • eukaryotic elongation factor-2 kinase
  • HSU93850
  • MGC45041

Background

The activity of the purified eukaryotic elongation-factor-2 kinase (eEF2K) is completely dependent on calcium and calmodulin, and autophosphorylation on serine and threonine residues is calcium/calmodulin-dependent. eEF2K is a ubiquitous protein kinase that phosphorylates and inactivates eEF2, and thus can modulate the rate of polypeptide chain elongation during translation. eEF2K is 120 kDa protein that is detected in skeletal muscle extracts and is phosphorylated rapidly by SAPK4, but poorly by p38, p38g, JNK or ERK 2. SAPK4 phosphorylates eEF2K at Ser 359 and Ser 396 in vitro, causing its inactivation. The phosphorylation of eEF2K at Ser 359 is also induced by insulin-like growth factor-1. Ser 359 is in close proximity to Ser 366 and the Ser 366 residue also becomes phosphorylated in response to growth factors. eEF2K is phosphorylated by p70 S6 kinase at Ser 366 and this results in the inactivation of eEF2K, especially at low (micromolar) calcium concentrations.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol EEF2K