EEF2K Antibody

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Western Blot: EEF2K Antibody [NBP1-32673] - Sample (30 ug of whole cell lysate) A: Hela S37C 5% SDS PAGE, antibody diluted at 1:1000.

Product Details

Summary
Reactivity Hu, RtSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

EEF2K Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human eEF-2K. The exact sequence is proprietary.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
EEF2K
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
Theoretical MW
82 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Mouse (88%), Bovine (89%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7.0), 0.1M Glycine and 10% Glycerol
Preservative
0.01% Thimerosal
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for EEF2K Antibody

  • Calcium/calmodulin-dependent eukaryotic elongation factor 2 kinase
  • calcium/calmodulin-dependent eukaryotic elongation factor-2 kinase
  • EC 2.7.11
  • EC 2.7.11.20
  • eEF-2 kinase
  • eEF-2Kcalmodulin-dependent protein kinase III
  • elongation factor-2 kinase
  • eukaroytic elongation factor 2 kinase
  • eukaryotic elongation factor 2 kinase
  • eukaryotic elongation factor-2 kinase
  • HSU93850
  • MGC45041

Background

Eukaryotic elongation factor-2 kinase (eEF-2k) previously known as Ca2+/calmodulin-dependent protein kinase III, is an abundant cytoplasmic protein highly specific for elongation factor-2 (eEf-2) (1). Phosphorylation of eEF-2 by eEF-2 kinase on specific threonine residues results in the inactivation of eEF-2 and in termination of mRNA translation (2). The activity of eEF2 kinase is not only dependent upon Ca2+ ions, calmodulin (CaM) and insulin, but is also regulated both negatively and positively via phosphorylation by different protein kinases (AMPK, S6K1, p90 RSK) (3-4). There is also evidence that eEF-2 phosphorylation is involved in the regulation of cell cycle progression, cellular differentiation, oogensis and malignant tumors (5-6).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol EEF2K