EED Antibody (671)

Images

 
Western Blot: EED Antibody (671) [NBP2-14940] - A. 30 ug Rat-2 whole cell lysate/extract 10 % SDS-PAGEEED antibody [671] dilution: 1:1000
Western Blot: EED Antibody (GT671) [NBP2-14940] - Sample (30 ug of whole cell lysate) A: 293T B: HeLa C: HepG2 10% SDS PAGE; antibody diluted at 1:5000.
Western Blot: EED Antibody (GT671) [NBP2-14940] - A. Neuro2A whole cell lysate/extract. B. GL261 whole cell lysate/extract. C. C8D30 whole cell lysate/extract. D.NIH-3T3 whole cell lysate/extract. E. C2C12 whole cell ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Bv, Ch, Mk, Xp, ZeSpecies Glossary
Applications WB
Clone
671
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

EED Antibody (671) Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human EED. The exact sequence is proprietary.
Predicted Species
Xenopus (96%), Zebrafish (93%), Monkey (100%), Bovine (100%), Chicken (99%). Backed by our 100% Guarantee.
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
EED
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000-1:10000
Theoretical MW
50 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Japanese medaka (95%), Xenopus laevis (96%), Rhesus Monkey (100%). Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
No Preservative
Concentration
1 mg/ml
Purity
Protein G purified

Alternate Names for EED Antibody (671)

  • EED
  • embryonic ectoderm development
  • HEED
  • polycomb protein EED
  • WAIT1
  • WAIT1
  • WAIT-1
  • WD protein associating with integrin cytoplasmic tails 1

Background

This gene encodes a member of the Polycomb-group (PcG) family. PcG family members form multimeric protein complexes, which are involved in maintaining the transcriptional repressive state of genes over successive cell generations. This protein interacts with enhancer of zeste 2, the cytoplasmic tail of integrin beta7, immunodeficiency virus type 1 (HIV-1) MA protein, and histone deacetylase proteins. This protein mediates repression of gene activity through histone deacetylation, and may act as a specific regulator of integrin function. Two transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

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Blogs on EED.

EZH1 has more to offer than gene repression
EZH1 is part of the Polycomb-group family of proteins, which are responsible for remodeling chromatin in genes and modulating epigenetic silencing during development.  Specifically, EZHI is a component of PRC2, or polycomb repressive complex-2.  PR...  Read full blog post.

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Bioinformatics

Gene Symbol EED
Entrez