EEA1 Antibody

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Western Blot: EEA1 Antibody [NBP1-05963] - Whole cell lysate from HeLa, 293T, and mouse NIH3T3 cells. EEA1 was immunoprecipitated by rabbit anti-EEA1 antibodies NBP1-05961 and NBP1-05962.

Product Details

Summary
Product Discontinued
View other related EEA1 Primary Antibodies

Order Details


    • Catalog Number
      NBP1-05963
    • Availability
      Product Discontinued

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EEA1 Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residue 1361 and 1411 of human early endosome antigen 1 using the numbering given in entry NP_003557.2 (GeneID 8411).
Marker
Early Endosome Marker
Clonality
Polyclonal
Host
Rabbit
Gene
EEA1
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Western Blot 1:2000-1:10000
Application Notes
This antibody is useful for western blot. It is not recommended for immunoprecipitation.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
TBS and 0.1% BSA
Preservative
0.09% Sodium Azide
Concentration
0.2 mg/ml
Purity
Immunogen affinity purified

Alternate Names for EEA1 Antibody

  • early endosome antigen 1
  • early endosome antigen 1, 162kD
  • early endosome-associated protein
  • EEA1
  • Endosome-associated protein p162
  • MST105
  • MSTP105
  • ZFYVE2
  • ZFYVE2MST105
  • Zinc finger FYVE domain-containing protein 2

Background

Early endosomal autoantigen 1 (EEA1) bears an FYVE (Fab1p-YOPB-Vps27p-EEA1) domain which is an approximately 80 amino acid double-zinc binding motif that binds to PtdIns(3)P (phosphatidylinositol 3-phosphate). The FYVE domain is found in proteins involved in intracellular signaling, membrane trafficking, and cytoskeletal rearrangement. EEA1 binds to early endosomal membranes that contain PtdIns(3)P and plays a role in the docking and fusion of early endosomes. Alternative names for EEA1 include endosome-associated protein p162, Zinc finger FYVE domain-containing protein 2, and ZFYVE2.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

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Secondary Antibodies

 

Isotype Controls

Additional EEA1 Products

Bioinformatics Tool for EEA1 Antibody (NBP1-05963)

Discover related pathways, diseases and genes to EEA1 Antibody (NBP1-05963). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Diseases for EEA1 Antibody (NBP1-05963)

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Pathways for EEA1 Antibody (NBP1-05963)

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PTMs for EEA1 Antibody (NBP1-05963)

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Research Areas for EEA1 Antibody (NBP1-05963)

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Blogs on EEA1.

Understanding EEA1's Role in Membrane Endosome Fusion
EEA1, or Early Endosome Antigen 1 is a Rab5 effector essential for early endocytic membrane fusion. The EEA1 antibody is used in membrane trafficking and chaperone studies, and as an endosome marker. We at Novus Biologicals have a comprehensive antibo...  Read full blog post.

EEA1 Antibodies in Endosomal Transport and Signalling Studies
EEA1, or Early Endosome Antigen 1, is a phospholipid-binding protein essential for endosomal membrane trafficking and fusion, and is a useful endosomal marker. We at Novus Biologicals have an extensive EEA1 antibody catalog, with antibodies that have...  Read full blog post.

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Bioinformatics

Gene Symbol EEA1
Entrez
Uniprot