EBOV Antibody

Immunocytochemistry/Immunofluorescence: EBOV Antibody [NBP3-14861] - Hela cells were transfected with plasmids expressing Ebola Zaire glycoprotein, nucleoprotein or VP40, seeded on coverslips and incubated for 48 h. Control coverslips consisted of untransfected cells. After fixation with 4% PFA, samples were stained with EBOV antibody. Antibody was used at a dilution of 1:500 and Triton X-100 was used as detergent. Imaging was performed using a Leica SP5 confocal microscope (Images by Virology Research Services Ltd).

• Reactivity: V
• Applications: WB, ELISA, ICC/IF
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

EBOV Antibody Summary

• Immunogen: Zaire ebola virus-like particle, recombinant protein
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/Immunofluorescence
  • Western Blot
• Application Notes: This antibody has been raised against recombinant EBOV virus like particles (VLPs), expressed in mammalian cells. These VLPs are comprised of EBOV Nucleoprotein (NP), glycoprotein (GP) and the VP40 protein. The antibody has been shown to react with each of the components of the VLP in Western blotting analysis, when used at a 1:2500 dilution.

Reactivity Notes

Ebola Virus

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS pH 7.4
• Preservative: No Preservative
• Concentration: 1 mg/ml
• Purity: Protein G purified

Background

Ebola virus is an enveloped, negative-stranded RNA virus. This virus belongs to the family Filoviridae and has a distinctive morphology similar only to that of Marburg virus, but apparently has no antigenic characteristics of Marburg virus. Its genome is a nonsegmented RNA strand. SDS-PAGE profiles demonstrate the presence of seven structural proteins. These are the major nucleoprotein NP (104 K), glycoprotein GP (125K), L protein (180 K), and four proteins: VP40 (40 K), VPX (35 K), VP30 (30 K) and VP24 (24 K). All the proteins are encoded by monocistronic mRNA transcripts complementary to virion RNA. GP is the major protein of the surface spikes and only this 125K viral protein is glycosylated.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.