EARS2 293T Cell Transient Overexpression Lysate

Images

 
Lane 1: EARS2 transfected lysate (57.64 KDa) Lane 2: Non-transfected lysate.
EARS2 transfected lysate.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

EARS2 293T Cell Transient Overexpression Lysate Summary

Description
Plasmid: pCMV-EARS2 full-length
Specificity
EARS2 293T Cell Transient Overexpression Lysate(Denatured)
Gene
EARS2

Applications/Dilutions

Dilutions
  • Western Blot

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)

Lysate Details for Array

Type
293T Cell Transient Overexpression
Protein State
Denatured

Notes

Quality control test: Transient overexpression cell lysate was tested with Anti-EARS2 antibody by Western Blots.

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for EARS2 293T Cell Transient Overexpression Lysate

  • EC 6.1.1.17
  • gluRS
  • Glutamate--tRNA ligase
  • glutamyl-tRNA synthetase 2, mitochondrial (putative)
  • KIAA1970glutamate tRNA ligase 2, mitochondrial
  • MSE1
  • probable glutamyl-tRNA synthetase, mitochondrial

Background

EARS2 catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction: tyrosine is first activated by ATP to form Tyr-AMP and then transferred to the acceptor end of tRNA(Tyr)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

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Bioinformatics

Gene Symbol EARS2