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Rat EAAT3 ELISA Kit (Colorimetric)

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ELISA: Rat EAAT3 ELISA Kit (Colorimetric) [NBP2-82471] - Standard Curve Reference
ELISA: Rat EAAT3 ELISA Kit (Colorimetric) [NBP2-82471] - Standard Curve Reference

Product Details

Summary
Reactivity RtSpecies Glossary
Applications ELISA
Suitable Sample Type
Serum, plasma, cell supernatant, and other biological fluids.
Standard Curve Range
0.63 - 40 ng/mL
Sensitivity
0.38 ng/mL

Order Details

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Rat EAAT3 ELISA Kit (Colorimetric) Summary

Specificity
This kit recognizes Rat EAAT3 in samples. No Significant cross-reactivity or interference between Rat EAAT3 and analogues was observed.
Standard Curve Range
0.63 - 40 ng/mL
Sensitivity
0.38 ng/mL
Assay Type
Sandwich-ELISA
Inter-Assay
CV% < 4.77
Intra-Assay
CV% < 5.39
Spike Recovery
85-102%
Sample Volume
100 ul
Kit Type
ELISA Kit (Colorimetric)
Gene
SLC1A1

Applications/Dilutions

Dilutions
  • ELISA

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Biotinylated Detection Ab Diluent
  2. Certificate of Analysis
  3. Concentrated Biotinylated Detection Ab (100x)
  4. Concentrated HRP Conjugate (100x)
  5. Concentrated Wash Buffer (25x)
  6. HRP Conjugate Diluent
  7. Micro ELISA Plate(Dismountable)
  8. Plate Sealer
  9. Product Description
  10. Reference Standard & Sample Diluent
  11. Reference Standard
  12. Stop Solution
  13. Substrate Reagent

Alternate Names for Rat EAAT3 ELISA Kit (Colorimetric)

  • EAAC1excitatory amino acid carrier 1
  • EAAT3excitatory amino acid transporter 3
  • Excitatory amino-acid carrier 1
  • Neuronal and epithelial glutamate transporter
  • Sodium-dependent glutamate/aspartate transporter 3
  • solute carrier family 1 (neuronal/epithelial high affinity glutamatetransporter, system Xag), member 1
  • Solute carrier family 1 member 1

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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NB100-1869
Flow Cytometry: EAAT1/GLAST-1/SLC1A3 Antibody [NB100-1869] - An intracellular stain was performed on Hek293 cells with EAAT1/GLAST-1/SLC1A3 antibody NB100-1869PE (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Phycoerythrin.
Flow Cytometry: EAAT1/GLAST-1/SLC1A3 Antibody [NB100-1869] - An intracellular stain was performed on Hek293 cells with EAAT1/GLAST-1/SLC1A3 Antibody NB100-1869AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.
Flow Cytometry: EAAT1/GLAST-1/SLC1A3 Antibody [NB100-1869] - An intracellular stain was performed on U937 cells with SLC1A3 Antibody NB100-1869 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1.0 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (SA5-10033, Thermo Fisher).
(1 Review)
(20 Publications)
Species: Hu, Mu, Rt
Applications: ELISA, Flow-IC, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, WB
EAAT2/GLT1
Antibody - Azide Free
NBP1-20136
Western Blot: EAAT2/GLT1 Antibody - Azide Free [NBP1-20136] - Blocking: 1% LFDM for 30 min at RT; primary antibody: dilution 1:1000 incubated overnight at 4C.
Immunohistochemistry-Paraffin: EAAT2/GLT1 Antibody - Azide Free [NBP1-20136] - Mouse spinal cord. The animal was perfused using Autoperfuser at a pressure of 130 mmHg with 300 ml 4% FA before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min. Blocking: 0.2% LFDM in TBST filtered thru 0.2 um.
Immunohistochemistry-Paraffin: EAAT2/GLT1 Antibody - Azide Free [NBP1-20136] - Mouse spinal cord. The animal was perfused using Autoperfuser at a pressure of 130 mmHg with 300 ml 4% FA before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20. Blocking: 0.2% LFDM in TBST filtered thru 0.2 um.
(9 Publications)
Species: Hu, Pm, Mu, Rt
Applications: Flow, ICC/IF, IHC, IHC-P, In vivo, ISH, WB
EAAT4 Antibody
NBP2-48740
Immunohistochemistry-Paraffin: EAAT4 Antibody [NBP2-48740] - Staining of human cerebral cortex shows high expression.
Immunohistochemistry-Paraffin: EAAT4 Antibody [NBP2-48740] - Staining of human kidney shows low expression as expected.
Immunocytochemistry/Immunofluorescence: EAAT4 Antibody [NBP2-48740] - Staining of human cell line A-431 shows localization to intermediate filaments.
Species: Hu
Applications: ICC/IF, IHC, IHC-P
Complement C6
Antibody
NBP1-91803
Western Blot: Complement C6 Antibody [NBP1-91803] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp. Lane 4: Human plasma (IgG/HSA depleted)
Immunohistochemistry-Paraffin: Complement C6 Antibody [NBP1-91803] - Staining of human prostate shows strong granular cytoplasmic and membranous positivity in glandular cells.
Species: Hu
Applications: IHC, IHC-P, WB
PSMA7 Antibody
(1A10-3G12)
H00005688-M01
Western Blot: PSMA7 Antibody (1A10-3G12) [H00005688-M01] - Analysis of PSMA7 expression in transfected 293T cell line by PSMA7 monoclonal antibody (M01), clone 1A10-3G12.Lane 1: PSMA7 transfected lysate (Predicted MW: 27.9 KDa).Lane 2: Non-transfected lysate.
Immunocytochemistry/Immunofluorescence: PSMA7 Antibody (1A10-3G12) [H00005688-M01] - Analysis of monoclonal antibody to PSMA7 on HeLa cell. Antibody concentration 1 ~ 10 ug/ml.
Immunohistochemistry-Paraffin: PSMA7 Antibody (1A10-3G12) [H00005688-M01] - Analysis of monoclonal antibody to PSMA7 on formalin-fixed paraffin-embedded human lung, adenosquamous cell carcinoma. Antibody concentration 5 ug/ml.
(1 Review)
(2 Publications)
Species: Hu
Applications: ELISA, ICC/IF, IHC, IHC-P, IP, S-ELISA, WB
JWA Antibody
NBP3-12158
Western Blot: JWA Antibody [NBP3-12158] - Staining of Human Cerebellum lysate with antibody at 1 ug/mL (35 ug protein in RIPA buffer). Detected by chemiluminescence.
Western Blot: JWA Antibody [NBP3-12158] - Staining of K562 cell lysate with antibody at 1 ug/mL (35 ug protein in RIPA buffer). Detected by chemiluminescence.
Immunocytochemistry/Immunofluorescence: JWA Antibody [NBP3-12158] - Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing plasma membrane and ER/cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
Species: Hu
Applications: Flow, ICC/IF, IHC, IHC-P, PEP-ELISA, WB
PRRT2 Antibody -
BSA Free
NBP2-82026
Western Blot: PRRT2 Antibody [NBP2-82026] - Analysis of PRRT2 in mouse brain tissue lysate with PRRT2 antibody at 1 ug/ml.
Immunohistochemistry: PRRT2 Antibody [NBP2-82026] - Immunohistochemistry of PRRT2 in rat brain tissue with PRRT2 antibody at 2.5 ug/ml.
Immunocytochemistry/Immunofluorescence: PRRT2 Antibody [NBP2-82026] - Immunofluorescence of PRRT2 in rat brain tissue with PRRT2 antibody at 20 ug/ml.
Species: Hu, Mu, Rt
Applications: ELISA, ICC/IF, IHC, IHC-P, WB
SLC1A7 Antibody -
BSA Free
NBP2-81802
Western Blot: SLC1A7 Antibody [NBP2-81802] - Analysis of SLC1A7 in C2C12 cell lysate with SLC1A7 antibody at 1 ug/ml in (A) the absence and (B) the presence of blocking peptide.
Immunohistochemistry: SLC1A7 Antibody [NBP2-81802] - Immunohistochemistry of SLC1A7 in mouse kidney tissue with SLC1A7 antibody at 5 ug/ml.
Immunocytochemistry/Immunofluorescence: SLC1A7 Antibody [NBP2-81802] - Immunofluorescence of SLC1A7 in mouse kidney tissue with SLC1A7 antibody at 20 ug/ml.
Species: Hu, Mu
Applications: ELISA, ICC/IF, IHC, IHC-P, WB
Carbonic Anhydrase
I/CA1 Antibody
NBP1-88191
Western Blot: Carbonic Anhydrase I/CA1 Antibody [NBP1-88191] - Analysis in human cell line HELA.
Immunohistochemistry-Paraffin: Carbonic Anhydrase I/CA1 Antibody [NBP1-88191] - Analysis in human rectum and pancreas tissues. Corresponding CA1 RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: Carbonic Anhydrase I/CA1 Antibody [NBP1-88191] - Staining of human duodenum shows no positivity in glandular cells as expected.
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Applications: IHC, IHC-P, WB
Glutamine
Synthetase Antibody
NB110-41404
Immunocytochemistry/Immunofluorescence: Glutamine Synthetase Antibody [NB110-41404] - Immunofluorescence using NB110-41404. Submitted via verified customer review.
Western Blot: Glutamine Synthetase Antibody [NB110-41404] - Analysis of glutamine synthase. 40ug of lysates from mouse (Lanes M), rat (Lane R), pig (Lane P), bovine (Lane B), or human (Lane Hu) retina were probed. A 42 kDa band was identified in lysates from retinas of all species.
Immunohistochemistry: Glutamine Synthetase Antibody [NB110-41404] - Localization of glutamine synthase in the retina. Paraffin sections of mouse (A, B), rat (C, D, G-I), or human (E, F) retina fixed in 4% paraformaldehyde were reacted with anti-glutamine synthase (red fluorescence staining in B, D, G, I, and brown immuno-peroxidase reaction. Nuclei in IF experiments (A-D) were stained with DAPI (cyan), and with nuclear fast red in E and F. At low magnification, anti-glutamine synthase reacted with a single population of cells extending from the ganglion cell layer through the inner nuclear layer. No signal was detected in controls either pre-incubated with 100ug/ml of the immunizing peptide (A) or with pre-immune serum (C, E). This finding was confirmed by co-localization (indicated by yellow in I) of glutamine synthase (red in G) with antoher marker of glutamine synthase (green in H).
(1 Review)
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GFAP Antibody
NB300-141
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Western Blot: GFAP Antibody [NB300-141] - Analysis of different tissue lysates using rabbit polyclonal antibody to GFAP, NB300-141, dilution 1:5000 in green: [1] protein standard (red), [2] rat brain, [3] rat spinal cord, [4] mouse brain, [5] mouse spinal cord. Strong band at about 50 kDa corresponds to the major isotype of the GFAP protein. Smaller isotypes and proteolytic fragments of GFAP are also detected on the blot.
Western Blot: GFAP Antibody [NB300-141] - Analysis of Rat brain lysate. Antibody at 1:5000. Specific band running with an apparent SDS-PAGE molecular weight of ~50 kDa corresponds to rodent GFAP was observed.
(12 Reviews)
(79 Publications)
Species: Bv, Ch, Eq, Gp, Hu, Mu, Po, Rb, Rt
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VGLUT1 Antibody
NBP3-13165
Immunohistochemistry-Paraffin: VGLUT1 Antibody [NBP3-13165] - VGLUT1 antibody detects VGLUT1 protein at cell membrane in rat brain by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. VGLUT1 antibody (NBP3-13165) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Immunohistochemistry-Frozen: VGLUT1 Antibody [NBP3-13165] - VGLUT1 antibody detects VGLUT1 protein expression by immunohistochemical analysis. Sample: Frozen-sectioned adult mouse cerebellum. Green: VGLUT1 protein stained by VGLUT1 antibody (NBP3-13165) diluted at 1:250. Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (NBP2-43559) diluted at 1:500. Blue: Fluoroshield with DAPI.
Western Blot: VGLUT1 Antibody [NBP3-13165] - Various tissue extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with VGLUT1 antibody (NBP3-13165) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
Species: Mu, Rt
Applications: ICC/IF, IHC, IHC-Fr, IHC-P, WB
mGluR2/3 Antibody
NB300-124
Western Blot: metabotropic Glutamate Receptor 2/3 Antibody [NB300-124] - Western blot of 10 ug of HEK 293 cells expression. As shown in the autoradiograph, the mGluR2/3 antibody recognizes both the ~110k monomer and the ~220k dimer of mGluR2 (Lane A) and mGluR3 (Lane B). Also shown is the specific immunolabeling of the monomer
Western Blot: metabotropic Glutamate Receptor 2/3 Antibody [NB300-124] - Specificity of mGluR2/3 (NB 300-124) in rat tissue lysate.
Western Blot: metabotropic Glutamate Receptor 2/3 Antibody [NB300-124] - Western blot of 10 ug of rat hippocampal lysate showing the specific immunolabeling of the ~110k monomer and the ~220k dimer of mGluR2 and mGluR3.
(5 Publications)
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PEPT1/SLC15A1
Antibody
NBP2-98544
Immunocytochemistry/Immunofluorescence: PEPT1/SLC15A1 Antibody [NBP2-98544] - Immunofluorescence staining of PEPT1/SLC15A1 in CACO-2 cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human PEPT1/SLC15A1 polyclonal antibody (dilution ratio 1:300) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Nucleus.
Immunohistochemistry-Paraffin: PEPT1/SLC15A1 Antibody [NBP2-98544] - Staining of human PEPT1/SLC15A1 in human gallbladder with rabbit polyclonal antibody at 1:500 dilution.
Immunohistochemistry-Paraffin: PEPT1/SLC15A1 Antibody [NBP2-98544] - Staining of human SLC15A1 in human small intestine with rabbit polyclonal antibody at 1:500 dilution.
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GluR2 Antibody
NBP1-89765
Immunohistochemistry-Paraffin: GluR2 Antibody [NBP1-89765] - Staining of human cerebral cortex shows high expression.
Immunohistochemistry-Paraffin: GluR2 Antibody [NBP1-89765] - Staining of human pancreas shows low expression as expected.
Immunohistochemistry-Paraffin: GluR2 Antibody [NBP1-89765] - Analysis in human cerebral cortex and liver tissues using NBP1-89765 antibody. Corresponding GRIA2 RNA-seq data are presented for the same tissues.
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PIK3CA Antibody
NBP2-19804
Western Blot: PIK3CA Antibody [NBP2-19804] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with PI3 kinase p110 alpha antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
Immunohistochemistry-Paraffin: PIK3CA Antibody [NBP2-19804] - Human Breast Cancer, using PI3 kinase p110 alpha antibody. Antigen Retrieval: Trilogy&amp;#8482; (EDTA based, pH 8.0) buffer, 15min.
Western Blot: PIK3CA Antibody [NBP2-19804] - PIK3CA Antibody Sample (50 ug of whole cell lysate) A: C2C12 B: C2C12+AEA10mM NBP2-19804 diluted at 1:1000.
(3 Publications)
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Applications: ICC/IF, IHC, IHC-P, WB
PI 3-Kinase p110
beta/PIK3CB Antibody
NBP1-33116
Immunocytochemistry/Immunofluorescence: PI 3-Kinase p110 beta/PIK3CB Antibody [NBP1-33116] - Analysis of methanol-fixed HeLa, using antibody at 1:200 dilution.
Western Blot: PI 3-Kinase p110 beta/PIK3CB Antibody [NBP1-33116] - Sample (50 ug of whole cell lysate) A: C2C12 B: C2C12+AEA10mM antibody diluted at 1:1000.
Western Blot: PI 3-Kinase p110 beta/PIK3CB Antibody [NBP1-33116] - Sample (30 ug of whole cell lysate) A: H1299 7.5% SDS PAGE; antibody diluted at 1:1000.
(2 Publications)
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Applications: ICC/IF, IHC, IHC-P, WB
Rat EAAT3 ELISA
Kit (Colorimetric)
NBP2-82471
Rat EAAT3 ELISA Kit (Colorimetric)
Species: Rt
Applications: ELISA

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Immunohistochemistry: GFAP Antibody [NB300-141] - Analysis of a rat cerebellum section stained with rabbit polyclonal antibody to GFAP, NB300-141, dilution 1:5000 in green and mouse monoclonal antibody to MeCP2, dilution 1:500, in red. The blue is DAPI staining of nuclear DNA. Following transcardial perfusion of rat with 4% paraformaldehyde, brain was post fixed for 1 hour, cut to 45 uM, and free-floating sections were stained with above antibodies. The GFAP antibody stains the network of astrocytic cells and the processes of Bergmann glia in the molecular layer. The MeCP2 antibody specifically labels nuclei of certain neurons.
GFAP Antibody
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Flow Cytometry: EAAT1/GLAST-1/SLC1A3 Antibody [NB100-1869] - An intracellular stain was performed on Hek293 cells with EAAT1/GLAST-1/SLC1A3 antibody NB100-1869PE (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Phycoerythrin.
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  Rat EAAT3 ELISA Kit (Colorimetric) Manual
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Bioinformatics

Gene Symbol SLC1A1