EAAT1/GLAST-1/SLC1A3 Antibody

Images

 
Western Blot: EAAT1/GLAST-1/SLC1A3 Antibody [NBP2-11258] - A) and hippocampal membranes (B). Apparent MW of Slc1A is 71kDa. Antibody dilution 1:500 in Diluition Buffer.

Product Details

Summary
Product Discontinued
View other related EAAT1/GLAST-1/SLC1A3 Primary Antibodies

Order Details


    • Catalog Number
      NBP2-11258
    • Availability
      Product Discontinued

    Can't find what you are looking for? Use our Antibody Concierge Service & we will help you locate your antibody!

    Or feel free to contact us for alternative products.

EAAT1/GLAST-1/SLC1A3 Antibody Summary

Immunogen
Synthetic peptide taken from human Slc1A protein taken from within the C-terminal domain of Slc1A. The peptide was conserved in several other species. The selected peptide was post-synthetically modified to achieve highest antigenicity before coupling to carrier protein using hetero bifunctional cross linker for immunogen preparation.
Epitope
aa: ckvtlaangksadcsveeepwkrek
Specificity
The antibody labels a strong diffuse band of Slc1A 71kDa in samples and in hippocampal membranes. The antibody also labels a weak band of 42kDa. The identity of this band is not known.
NP_004162
Clonality
Polyclonal
Host
Rabbit
Gene
SLC1A3
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Conjugate
Unconjugated
Buffer
Tris/Glycine buffer pH 7.5-7.9, stabilizing protein, and glycerol
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Applications/Dilutions

Dilutions
  • Western Blot 1:500
  • ELISA 1:500
Application Notes
Slc1A antibody was tested in ELISA and western blotting applications

Reactivity Notes

Human, Mouse and Rat

Alternate Names for EAAT1/GLAST-1/SLC1A3 Antibody

  • EA6
  • EA6FLJ25094
  • EAAT1/GLAST-1
  • EAAT1GLAST-1
  • excitatory amino acid transporter 1
  • GLAST1
  • GLAST-1
  • GLASTGLAST1
  • SLC1A3
  • Sodium-dependent glutamate/aspartate transporter 1
  • solute carrier family 1 (glial high affinity glutamate transporter), member 3
  • Solute carrier family 1 member 3

Background

The Solute Carriers (SLCs) are membrane transport proteins that regulate transport of substances across the cell membrane such as sugars, amino acids, nucleotides, inorganic ions, and drugs. The Slc family members include genes that encode passive transporters, ion transporters, and exchangers. Slc transporter entirly rely on an ion gradient over the cell membrane as the driving force for transport across the membrane. The Slc's do not involve in primary active transport of solutes across the membrane (1). Excitatory amino acid transporters (EAATs) are high-affinity, sodium-dependent glutamate transport carriers from solute carrier family 1 (Slc1), primarily expressed in astroglia and are responsible for most of the glutamate transport in the adult brain (2). Glutamate transporters (the human homolog of which is excitatory amino acid transporter) are required in the termination of the synaptic actions of the neurotransmitter and keep its synaptic concentrations below neurotoxic levels. Glutamate transport is an electrogenic process consisting of two sequential translocation steps, co-transport of the neurotransmitter with three sodium ions and a proton and the counter-transport of one potassium ion. Glutamate transporters mediate substrate-induced steady-state current by the electrogenic transport. Non-transportable substrate analogs, expected to "lock" the transporter in an outward-facing conformation and are the competitive inhibitors of two types of substrate-induced current, and also inhibit the basal sodium-dependent anion conductance (3). The function of glutamate transporters is to regulate synaptic glutamate levels and to prevent excitotoxic neuronal death as a result of prolonged excitation of glutamate receptors. Slc1is a multi-trans-membrane-spanning protein that exist as multi subunit protein in membrane (4). Glutamate transporters are essential in preventing hyperexcitability and CNS neurotoxicity. Glutamate transporters (Slc1A) are responsible for clearing synaptically released glutamate at excitatory synapses. Slc1A reduce spillover, thus ensuring a spatially restricted action of glutamate and preventing its accumulation in the extracellular space. By these actions, Slc1A contribute to sculpting excitatory postsynaptic currents and to modulating synaptic plasticity. Slc1A isoform, exhibits the highest level of expression and is responsible for the largest proportion of glutamate transport in hippocampus. Slc1A down-regulation increases extracellular glutamate levels which in turn cause excitotoxicity, conversely, an elevated expression of Slc1A may be neuroprotective (5).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP1-20136
Species: Hu, Mu, Rt
Applications: WB, Flow, ICC/IF, IHC, IHC-P, In vivo
NBP1-84938
Species: Hu
Applications: ICC/IF, IHC, IHC-P
NB110-41404
Species: Hu, Mu, Rt, Bv
Applications: WB, IHC
NBP2-48740
Species: Hu
Applications: IHC
NB300-141
Species: Hu, Mu, Rt, Po, Bv, Ca, Ch, Fe, GP, Rb
Applications: WB, Simple Western, ICC/IF, IHC, IHC-Fr, IHC-P
NBP2-13317
Species: Hu
Applications: IHC, IHC-P
NB100-91662
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
AF2180
Species: Hu
Applications: WB, Simple Western, IP
MAB1259
Species: Hu
Applications: ICC, ICFlow
NB300-124
Species: Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-Fr
NBP2-27073
Species: Hu, Mu, Bv, Eq, Op, Pm
Applications: WB, IHC-P
NBP2-46627
Species: Hu, Mu, Rt
Applications: WB, IHC, IHC-P
NB300-223
Species: Hu, Mu, Rt, Ca
Applications: WB, ICC/IF, IHC, IHC-Fr, IHC-P
MAB45141
Species: Hu
Applications: IHC

Publications for EAAT1/GLAST-1/SLC1A3 Antibody (NBP2-11258) (0)

There are no publications for EAAT1/GLAST-1/SLC1A3 Antibody (NBP2-11258).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for EAAT1/GLAST-1/SLC1A3 Antibody (NBP2-11258) (0)

There are no reviews for EAAT1/GLAST-1/SLC1A3 Antibody (NBP2-11258). By submitting a review earn points towards our Rewards Program.
  • 250 points for product review
  • 500 additional points for an image with your product review
  • Double points (500) if you are the first to review this product
  • Double points (1000) if you are the first to review this product with an image

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for EAAT1/GLAST-1/SLC1A3 Antibody (NBP2-11258) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional EAAT1/GLAST-1/SLC1A3 Antibody Products

Related Products by Gene

Bioinformatics Tool for EAAT1/GLAST-1/SLC1A3 Antibody (NBP2-11258)

Discover related pathways, diseases and genes to EAAT1/GLAST-1/SLC1A3 Antibody (NBP2-11258). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for EAAT1/GLAST-1/SLC1A3 Antibody (NBP2-11258)

Discover more about diseases related to EAAT1/GLAST-1/SLC1A3 Antibody (NBP2-11258).
 

Pathways for EAAT1/GLAST-1/SLC1A3 Antibody (NBP2-11258)

View related products by pathway.

PTMs for EAAT1/GLAST-1/SLC1A3 Antibody (NBP2-11258)

Learn more about PTMs related to EAAT1/GLAST-1/SLC1A3 Antibody (NBP2-11258).
 

Research Areas for EAAT1/GLAST-1/SLC1A3 Antibody (NBP2-11258)

Find related products by research area.

Blogs on EAAT1/GLAST-1/SLC1A3

There are no specific blogs for EAAT1/GLAST-1/SLC1A3, but you can read our latest blog posts.

Contact Information

Product PDFs

Bioinformatics

Gene Symbol SLC1A3
Entrez
Uniprot

Customer Resources

Novus Review - Submit your review and earn rewards points which can be used for merchandise & discounts.
Risk Free Testing - Test on a species/application not listed above to receive a full credit towards a future purchase.

Novus' Quality Guarantee - Novus guarantees that every product we sell will work in the application and species listed on our website and datasheets.

Submit your question on NBP2-11258 below.
During business hours, we will respond to your email within 24 hours. For any questions submitted on the weekend, a response will be received on Monday.
For immediate assistance during business hours M- F (excluding major holidays), please contact us.
Ask a Scientist - We have a lab full of white coats just waiting for your scientific questions and concerns.