E2F5 Antibody

Images

 
Western Blot: E2F5 Antibody [NBP1-51239] - Analysis of E2F-5 (L142) pAb at 1:500 dilution Lane1:HepG2 whole cell lysate(40ug) Lane2:NIH-3T3 whole cell lysate(40ug) Lane3:H9C2 whole cell lysate(40ug)
Western Blot: E2F5 Antibody [NBP1-51239] - Analysis of E2F-5 (L142) pAb in extracts from HeLa cells.

Product Details

Summary
Product Discontinued
View other related E2F5 Primary Antibodies

Order Details


    • Catalog Number
      NBP1-51239
    • Availability
      Product Discontinued

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E2F5 Antibody Summary

Immunogen
Synthetic peptide, corresponding to amino acids 110-166 of Human E2F-5.
Specificity
E2F-5 (L142) pAb detects endogenous levels of E2F-5 protein.
Clonality
Polyclonal
Host
Rabbit
Gene
E2F5
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:1000
Application Notes
Western blot (WB) analysis of E2F-5 (L142) pAb in extracts from HeLa cells.
Theoretical MW
36 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publication using
NBP1-51239 in the following applications:

  • WB
    1 publication

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2)
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for E2F5 Antibody

  • E2F transcription factor 5, p130-binding
  • E2F-5
  • transcription factor E2F5

Background

The human retinoblastoma gene product appears to play an important role in the negative regulation of cell proliferation. Functional inactivation of Rb can be mediated either through mutation or as a consequence of interaction with DNA tumor virus encoded proteins. Of all the Rb associations described to date, the identification of a complex between Rb and the transcription factor E2F most directly implicates Rb in regulation of cell proliferation. E2F was originally identified through its role in transcriptional activation of the adenovirus E2 promoter. Sequences homologous to the E2F binding site have been found upstream of a number of genes that encode proteins with putative functions in the G1 and S phases of the cell cycle. E2F-1 is a member of a broader family of transcription regulators including E2F-2, E2F-3, E2F-4, E2F-5 and E2F-6, each of which forms heterodimers with a second protein, DP-1, forming an "active" E2F transcriptional regulatory complex.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for E2F5 Antibody (NBP1-51239)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: WB.


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WB
(1)
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Human
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Secondary Antibodies

 

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Bioinformatics

Gene Symbol E2F5
Entrez
Uniprot