E2F4/E2F5 Antibody

Product Discontinued

E2F4/E2F5 Antibody Summary

• Immunogen: Synthetic peptide, corresponding to amino acids 18-72 of Human E2F4.
• Clonality: Polyclonal
• Host: Rabbit
• Gene: E2F4
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:500-1:1000
• Application Notes: Western blot (WB) analysis of E2F4/E2F5 (L50/83) pAb in extracts from COS7 cells.
  Immunohistochemistry (IHC) analyzes of E2F4/E2F5 (L50/83) pAb in paraffin-embedded human lung carcinoma tissue.
• Theoretical MW: 44 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  E2F-4 Overexpression Lysate

Reactivity Notes

Human, Mouse and Rat.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2)
• Preservative: 0.05% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for E2F4/E2F5 Antibody

• E2F transcription factor 4, p107/p130-binding
• E2F-4p107/p130-binding protein
• transcription factor E2F4

Background

The human retinoblastoma gene product appears to play an important role in the negative regulation of cell proliferation. Functional inactivation of Rb can be mediated either through mutation or as a consequence of interaction with DNA tumor virus encoded proteins. Of all the Rb associations described to date, the identification of a complex between Rb and the transcription factor E2F most directly implicates Rb in regulation of cell proliferation. E2F was originally identified through its role in transcriptional activation of the adenovirus E2 promoter. Sequences homologous to the E2F binding site have been found upstream of a number of genes that encode proteins with putative functions in the G1 and S phases of the cell cycle. E2F-1 is a member of a broader family of transcriptional regulators including E2F-2, E2F-3, E2F-4, E2F-5 and E2F-6, each of which forms heterodimers with a second protein, DP-1, forming an

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: E2F4
• Entrez:
  • 1874(Human)
  • 104394(Mouse)
  • 307794(Rat)
• Uniprot:
  • Q16254()