E2F-4 Antibody (SPM179)

Product Discontinued

E2F-4 Antibody (SPM179) Summary

• Immunogen: Recombinant DP1 protein.
• Epitope: Not determined.
• Localization: Nuclear
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: E2F4
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:50
  • Western Blot 1-2 ug/ml
• Application Notes: This antibody may be useful for Immunofluorescence (PMID: 22009766).
• Control:

  E2F-4 Overexpression Lysate
  Human Tonsil Whole Tissue Lysate (Adult Whole Normal)

Reactivity Notes

Human and Rat.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 10mM PBS (pH 7.4) and BSA
• Preservative: Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Protein G purified

Alternate Names for E2F-4 Antibody (SPM179)

• E2F transcription factor 4, p107/p130-binding
• E2F4
• E2F-4
• E2F-4p107/p130-binding protein
• transcription factor E2F4

Background

E2Fs are DNA binding proteins, which associate with negative regulators, such as the retinoblastoma p107 protein, resulting in an altered rate of gene transcription. E2F4 is regulated by complex formation with Rb p110. E2F family members bind DNA as heterodimers with members of the DP family of polypeptides.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for E2F-4 Antibody (NB120-17845)(2)

Publications using NB120-17845

• Hanna JA, Wimberly H, Kumar S et al. Quantitative analysis of microRNAs in tissue microarrays by in situ hybridization. Biotechniques 2012-04-01 [PMID: 22482439] (IHC-P, Human)
• Anagnostou VK, Dimou AT, Botsis T et al. Molecular classification of nonsmall-cell lung cancer using a 4-protein quantitative assay. Cancer. 2011-08-25 [PMID: 22009766] (WB, Human)

Bioinformatics

• Gene Symbol: E2F4
• Entrez:
  • 1874(Human)
  • 307794(Rat)