E-Selectin/CD62E Antibody (ENA1) [Biotin]



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E-Selectin/CD62E Antibody (ENA1) [Biotin] Summary

The antibody reacts with human endothelial cells activated with TNF, IL-1 or endotoxin.
ENA1 reacts with E-selectin CD62-E, previous designated as the Endothelial Leucocyte Adhesion Molecule-1 (ELAM-1)[1-4]. The antibody reacts with human endothelial cells activated with TNF, IL-1 or endotoxin. The antibody was found to react also with cells transfected with the ELAM-1 gene. The antibody inhibits the adhesion of granulocytes both neutrophilic and eosinophilic.
Protein A or G purified
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Packaging, Storage & Formulations

Store at 4C. Do not freeze.
Biotin (A=N/A, E=N/A)
PBS and 0.1% BSA
0.02% Sodium Azide
Protein A or G purified


Application Notes
In vitro cultured cells can be fixed with 1% paraformaldehyde, tissue sections are advised to be fixed for 10 min. in pure acetone and followed by incubation for 10 min in chloroform. Incubation with a pretested dilution of the antibody is advised to be followed by a biotin conjugated anti murine Ig and a further incubation with an enzyme (alkaline phosphatase) conjugated streptavidin. For selection of the most useful dilution in a given situation a test staining with cells or tissue known to express the antigen should be performed. To this end either cultured endothelial cells or a small fresh skin biopsy can be incubated for 4 hours with TNF (1 ng/ml), IL-1 (100 u/ml) or LPS (1 µg/ml) in tissue culture medium at 37degreesC.

Reactivity Notes



CD62E represents the 100-110 kDa type I transmembrane glycoprotein E-selectin, also known as endothelial-leukocyte adhesion molecule-1 (ELAM-1). It is expressed on cytokine-stimulated endothelium, weakly on unactivated endothelium, and is absent on leukocytes. Like other selectins, CD62E is an adhesion molecule that contributes to the initial attachment and rolling of leukocytes on endothelial surfaces, a prerequisite for leukocyte extravasation into tissues. (1-4)


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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