E-Selectin/CD62E Antibody (ENA1)

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Product Details

Summary
Product Discontinued
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Order Details


    • Catalog Number
      NBP1-42278
    • Availability
      Product Discontinued

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E-Selectin/CD62E Antibody (ENA1) Summary

Immunogen
The antibody reacts with human endothelial cells activated with TNF, IL-1 or endotoxin.
Specificity
ENA1 reacts with E-selectin CD62-E, previous designated as the Endothelial Leucocyte Adhesion Molecule-1 (ELAM-1)[1-4]. The antibody reacts with human endothelial cells activated with TNF, IL-1 or endotoxin. The antibody was found to react also with cells transfected with the ELAM-1 gene. The antibody inhibits the adhesion of granulocytes both neutrophilic and eosinophilic.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
SELE
Purity
Protein A or G purified
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Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
Application Notes
The antibody is extremely useful for staining of ELAM-1 expressing endothelial cells. It permits staining of in vitro cultured cells as well as frozen sections. The antibody can also be used for staining of chimpanzee, rhesus monkey, cynomolgus monkey andIn vitro cultured cells can be fixed with 1% paraformaldehyde, tissue sections are advised to be fixed for 10 min. in pure acetone and followed by incubation for 10 min in chloroform. Incubation with a pretested dilution of the antibody is advised to be f

Reactivity Notes

Human

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
No buffer
Preservative
0.02% Sodium Azide
Purity
Protein A or G purified

Alternate Names for E-Selectin/CD62E Antibody (ENA1)

  • CD62 antigen-like family member E
  • CD62E antigen
  • CD62E
  • ELAM
  • ELAM1
  • ELAM-1
  • ELAM1E-selectin
  • endothelial adhesion molecule 1
  • Endothelial leukocyte adhesion molecule 1
  • ESEL
  • E-Selectin
  • LECAM2
  • leukocyte endothelial cell adhesion molecule 2
  • Leukocyte-endothelial cell adhesion molecule 2
  • SELE
  • selectin E

Background

CD62E represents the 100-110 kDa type I transmembrane glycoprotein E-selectin, also known as endothelial-leukocyte adhesion molecule-1 (ELAM-1). It is expressed on cytokine-stimulated endothelium, weakly on unactivated endothelium, and is absent on leukocytes. Like other selectins, CD62E is an adhesion molecule that contributes to the initial attachment and rolling of leukocytes on endothelial surfaces, a prerequisite for leukocyte extravasation into tissues. (1-4)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol SELE
Entrez