E-Cadherin Antibody (3F4)

Images

 
Western Blot: E-Cadherin Antibody (3F4) [H00000999-M01] - CDH1 monoclonal antibody (M01), clone 3F4. Analysis of CDH1 expression in human kidney.
Immunohistochemistry-Paraffin: E-Cadherin Antibody (3F4) [H00000999-M01] - Analysis of monoclonal antibody to CDH1 on formalin-fixed paraffin-embedded human stomach. Antibody concentration 0.375 ug/ml.
Western Blot: E-Cadherin Antibody (3F4) [H00000999-M01] - CDH1 expression in transfected 293T cell line by CDH1 monoclonal antibody (M01), clone 3F4. Lane 1: CDH1 transfected lysate (97.5 KDa). Lane 2: Non-transfected ...read more
Proximity Ligation Assay: E-Cadherin Antibody (3F4) [H00000999-M01] - Analysis of protein-protein interactions between EGFR and CDH1. HeLa cells were stained with anti-EGFR rabbit purified polyclonal 1:1200 and ...read more
Sandwich ELISA: E-Cadherin Antibody (3F4) [H00000999-M01] - Detection limit for recombinant GST tagged CDH1 is 0.1 ng/ml as a capture antibody.

Product Details

Summary
Reactivity Hu, PmSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC-P, PLA
Clone
3F4
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

E-Cadherin Antibody (3F4) Summary

Immunogen
CDH1 (NP_004351, 381 a.a. - 480 a.a.) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. KGQVPENEANVVITTLKVTDADAPNTPAWEAVYTILNDDGGQFVVTTNPVNNDGILKTAKGLDFEAKQQYILHVAVTNVVPFEVSLTTSTATVTVDVLDV
Marker
Plasma Membrane Marker
Specificity
CDH1 - cadherin 1, type 1, E-cadherin (epithelial)
Predicted Species
Primate (97%). Backed by our 100% Guarantee.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
CDH1
Purity
IgG purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot
  • ELISA
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry-Paraffin
  • Proximity Ligation Assay
Application Notes
Antibody reactivity against recombinant protein for WB. It has been used for IHC-P and ELISA. ICC/IF usage reported in scientific literature (PMID: 25612290).
Publications
Read Publications using
H00000999-M01 in the following applications:

Reactivity Notes

Immunogen sequence similarity with other species: Primate/Monkey (97%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4)
Preservative
No Preservative
Purity
IgG purified

Notes

Quality control test: Antibody Reactive Against Recombinant Protein.

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for E-Cadherin Antibody (3F4)

  • Arc-1
  • CAD1
  • cadherin 1, E-cadherin (epithelial)
  • cadherin 1, type 1, E-cadherin (epithelial)
  • Cadherin-1
  • calcium-dependent adhesion protein, epithelial
  • CAM 120/80
  • CD324 antigen
  • CD324
  • CDH1
  • CDHE
  • cell-CAM 120/80
  • Cell-CAM120/80
  • ECAD
  • ECadherin
  • E-Cadherin
  • Epithelial cadherin
  • LCAM
  • L-CAM
  • UVOE-Cadherin
  • Uvomorulin

Background

This gene is a classical cadherin from the cadherin superfamily. The encoded protein is a calcium dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Mutations in this gene are correlated with gastric, breast, colorectal, thyroid and ovarian cancer. Loss of function is thought to contribute to progression in cancer by increasing proliferation, invasion, and/or metastasis. The ectodomain of this protein mediates bacterial adhesion to mammalian cells and the cytoplasmic domain is required for internalization. Identified transcript variants arise from mutation at consensus splice sites.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for E-Cadherin Antibody (H00000999-M01)(2)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: ICC/IF.


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ICC/IF
(1)
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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

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Bioinformatics Tool for E-Cadherin Antibody (H00000999-M01)

Discover related pathways, diseases and genes to E-Cadherin Antibody (H00000999-M01). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Blogs on E-Cadherin.

Cathepsin B - a lysosomal protease with potential of an important drug target in neurological diseases and cancer
Cathepsins are a family of lysosomal proteases (serine, aspartic and cysteine proteases) that acts in conjunction with lipases and nucleases to degrade biological macromolecules in the lysosomes (1). While most cathepsins are ubiquitously expressed...  Read full blog post.

SOX2 - a stem cell transcription factor
The SOX gene family encodes a group of highly conserved transcription factors defined by the presence of a conserved high motility group (HMG) DNA-binding domain. They are involved in embryonic development regulation and cell fate determination. Al...  Read full blog post.

Beta-catenin - I am versatile!
Beta-catenin is a cytosolic, 88 kDa intracellular protein associated with cell surface cadherin glycoproteins. It is a member of the larger calcium-dependent catenin family that includes alpha-catenin, beta-catenin, and gamma-catenin (also known as pl...  Read full blog post.

Vimentin: Regulating EMT and Cancer
Vimentin, a member of the intermediate filament (IF) family, is a protein responsible for maintaining cellular integrity and reducing damage caused by stress. The vimentin protein is ubiquitously expressed in normal mesenchymal cells, and recent resea...  Read full blog post.

Carbonic Anhydrase IX Roles in Tumor Growth, Survival and Invasion
Carbonic anhydrase IX (CAIX) is a membrane-associated carbonic anhydrase, strongly induced by hypoxia. CA IX is overexpressed by several cancer cells from many tumor types, and is a component of the pH regulatory system invoked by these cells to comba...  Read full blog post.

E-Cadherin as a Cancer Biomarker
E-cadherin is a calcium-regulated adhesion molecule expressed in most normal epithelial tissues. E-cadherin is also associated with gland formation, stratification, and epithelial polarization, while loss of E-cadherin can cause dedifferentiation and...  Read full blog post.

E-Cadherin in Cell-Cell Adhesion and Cancer Diagnostics
E-Cadherin is a member of the cadherin superfamily and is fundamental player in a wide range of cellular processes such as development, morphology, polarity, migration and tissue integrity. Specifically, E-cadherin is an approximately 100 kDa epitheli...  Read full blog post.

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Bioinformatics

Gene Symbol CDH1
Entrez
OMIM
Uniprot