Western Blot: E-Cadherin Antibody (3F4) [H00000999-M01] - CDH1 monoclonal antibody (M01), clone 3F4. Analysis of CDH1 expression in human kidney.
Immunohistochemistry-Paraffin: E-Cadherin Antibody (3F4) [H00000999-M01] - Analysis of monoclonal antibody to CDH1 on formalin-fixed paraffin-embedded human stomach. Antibody concentration 0.375 ug/ml.
Western Blot: E-Cadherin Antibody (3F4) [H00000999-M01] - CDH1 expression in transfected 293T cell line by CDH1 monoclonal antibody (M01), clone 3F4. Lane 1: CDH1 transfected lysate (97.5 KDa). Lane 2: Non-transfected ...read more
Proximity Ligation Assay: E-Cadherin Antibody (3F4) [H00000999-M01] - Analysis of protein-protein interactions between EGFR and CDH1. HeLa cells were stained with anti-EGFR rabbit purified polyclonal 1:1200 and ...read more
Sandwich ELISA: E-Cadherin Antibody (3F4) [H00000999-M01] - Detection limit for recombinant GST tagged CDH1 is 0.1 ng/ml as a capture antibody.
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E-Cadherin Antibody (3F4) Summary
CDH1 (NP_004351, 381 a.a. - 480 a.a.) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. KGQVPENEANVVITTLKVTDADAPNTPAWEAVYTILNDDGGQFVVTTNPVNNDGILKTAKGLDFEAKQQYILHVAVTNVVPFEVSLTTSTATVTVDVLDV
Plasma Membrane Marker
CDH1 - cadherin 1, type 1, E-cadherin (epithelial)
Primate (97%). Backed by our 100% Guarantee.
Test in a species/application not listed above to receive a full credit towards a future purchase.
Immunogen sequence similarity with other species: Primate/Monkey (97%).
Packaging, Storage & Formulations
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
PBS (pH 7.4)
Quality control test: Antibody Reactive Against Recombinant Protein.
This product is produced by and distributed for Abnova, a company based in Taiwan.
Alternate Names for E-Cadherin Antibody (3F4)
cadherin 1, E-cadherin (epithelial)
cadherin 1, type 1, E-cadherin (epithelial)
calcium-dependent adhesion protein, epithelial
This gene is a classical cadherin from the cadherin superfamily. The encoded protein is a calcium dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Mutations in this gene are correlated with gastric, breast, colorectal, thyroid and ovarian cancer. Loss of function is thought to contribute to progression in cancer by increasing proliferation, invasion, and/or metastasis. The ectodomain of this protein mediates bacterial adhesion to mammalian cells and the cytoplasmic domain is required for internalization. Identified transcript variants arise from mutation at consensus splice sites.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
SOX2 - a stem cell transcription factor The SOX gene family encodes a group of highly conserved transcription factors defined by the presence of a conserved high motility group (HMG) DNA-binding domain. They are involved in embryonic development regulation and cell fate determination. Al... Read full blog post.
Beta-catenin - I am versatile! Beta-catenin is a cytosolic, 88 kDa intracellular protein associated with cell surface cadherin glycoproteins. It is a member of the larger calcium-dependent catenin family that includes alpha-catenin, beta-catenin, and gamma-catenin (also known as pl... Read full blog post.
Vimentin: Regulating EMT and Cancer Vimentin, a member of the intermediate filament (IF) family, is a protein responsible for maintaining cellular integrity and reducing damage caused by stress. The vimentin protein is ubiquitously expressed in normal mesenchymal cells, and recent resea... Read full blog post.
E-Cadherin as a Cancer Biomarker E-cadherin is a calcium-regulated adhesion molecule expressed in most normal epithelial tissues. E-cadherin is also associated with gland formation, stratification, and epithelial polarization, while loss of E-cadherin can cause dedifferentiation and... Read full blog post.
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