Dynamin Antibody (A1B2)

Western Blot: Dynamin Antibody (A1B2) [NBP2-80397] - Analysis of Dynamin on PC-3M cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (NBP2-80397, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.

Immunohistochemistry-Paraffin: Dynamin Antibody (A1B2) [NBP2-80397] - Analysis in mouse brain tissue using Dynamin antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with

Flow Cytometry: Dynamin Antibody (A1B2) [NBP2-80397] - Analysis of Dynamin 1 was done on A549 cells. The cells were fixed, permeabilized and stained with the primary antibody (1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Mouse IgG Secondary antibody at 1/1000 dilution for 30 minutes. Unlabeled sample was used as a control (cells without incubation with primary antibody; black).

Immunohistochemistry-Paraffin: Dynamin Antibody (A1B2) [NBP2-80397] - Analysis in rat brain tissue using Dynamin antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with dd

• Reactivity: Hu, Mu, Rt
• Applications: WB, Flow, IHC, IHC-P
• Clone: A1B2
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 2 mg/ml

Dynamin Antibody (A1B2) Summary

• Immunogen: Recombinant protein within human Dynamin aa 500-800. (SwissProt: Q05193 Human;SwissProt: P39053 Mouse;SwissProt: P21575 Rat)
• Localization: Cytoskeleton, cytoplasm.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: DNM1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50-1:100
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:500-1:2000
• Theoretical MW: 97 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any. Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-questions.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 1X TBS(pH7.4), 1%BSA, 50%Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 2 mg/ml
• Purity: Protein A purified

Alternate Names for Dynamin Antibody (A1B2)

• DNM
• DNM1
• dynamin 1
• Dynamin
• dynamin-1
• EC 3.6.5.5

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: DNM1