DYKDDDDK Epitope Tag Antibody

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Western Blot: DYKDDDDK Epitope Tag Antibody [NBP1-97391] - Used at a dilution of 1:2,500 to detect 1.0 ug of recombinant protein containing either the FLAG epitope tag linked at the carboxy (C) or the amino (N) terminus ...read more
Western Blot: DYKDDDDK Epitope Tag Antibody [NBP1-97391] - Used at a 1:1,000 dilution to detect 3-fold serial dilutions of amino-terminal FLAG Bacterial Alkaline Phosphatase (BAP) fusion protein starting at 1.0 ug of ...read more

Product Details

Summary
Product Discontinued
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Order Details


    • Catalog Number
      NBP1-97391
    • Availability
      Product Discontinued

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DYKDDDDK Epitope Tag Antibody Summary

Immunogen
This antibody was purified from whole rabbit serum prepared by repeated immunizations with the Enterokinase Cleavage Site (ECS) peptide DYKDDDDK (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys) conjugated to KLH using maleimide. Residues of glycine and cysteine were ad
Specificity

Each kit contains one unit of the following products: (100 ug)Anti-6X HIS EPITOPE TAG (RABBIT) Antibody (250 ug) Antibody for the detection of FLAG conjugated proteins (RABBIT) Antibody (100 ug)
Clonality
Polyclonal
Host
Rabbit
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Western Blot 1:2000-1:10000
  • ELISA 1:90000-1:250000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
Application Notes
This antibody is optimally suited for monitoring the expression of FLAG tagged fusion proteins. As such, this antibody can be used - identify fusion proteins containing the FLAG epitope. The antibody recognizes the epitope tag fused - either the amino- or carboxy- termini of targeted proteins. This antibody has been tested by ELISA and western blotting against both the immunizing peptide and FLAGacontaining recombinant proteins. Although not tested, this antibody is likely functional for immunoprecipitation, immunocytochemistry, and other immunodetection techniques. The epitope tag peptide sequence was first derived from the 11-amino-acid leader peptide of the gene-10 product from bacteriophage T7. Now the most commonly used hydrophilic octapeptide is DYKDDDDK.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for DYKDDDDK Epitope Tag Antibody

  • DDDDK epitope tag
  • DDK
  • DYKDDDDK Epitope Tag
  • DYKDDDDK tag
  • ECS epitope tag
  • ECS tag
  • Enterokinase Cleavage Site epitope tag
  • Enterokinase Cleavage Site tag
  • epitope tag flag
  • FLAG (FLAG is a trademark of Sigma Aldrich)
  • FLAG Tag
  • Ty 1 Tag
  • xxxDDDDK epitope tag

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for DYKDDDDK Epitope Tag Antibody (NBP1-97391). (Showing 1 - 6 of 6 FAQ).

  1. Are the FLAG Tag antibodies validated in Simple Western?
    • Yes, we offer a 2 FLAG Tag antibodies that have been tested in Simple Western: NB600-345 and NBP1-71705.
  2. Does FLAG Tag antibodies comes in lyophilized form?
    • The following FLAG Tag antibodies come in lyophilized form: NBP1-97401, NBP1-97399, NBP1-97400, NBP1-97402.
  3. What the theoretical molecular weight for FLAG Tag antibodies?
    • The TMW of FLAG Tag antibodies is approximately 150 kDa.
  4. Will FLAG Tag antibodies detect internal FLAG tags? My FLAG sequence is sandwiched between two other protein sequences.
    • If you are applying your sample(s) to SDS-PAGE, under reducing condition and follow by Western Blot, DYKDDDDK epitope tag antibodies should be able to detect the tag region located internally. However, it depends on the property of your chimeric protein and if your tag is in the hydrophobic or hydrophilic region. Although DYKDDDDK is very hydrophilic, it may not be exposed to the surface if embedded deeply inside the protein with a high content of hydrophobic amino acids with or without covalently-linked modifications such as lipid moieties.
  5. Will FLAG Tag antibodies recognizes FLAG at the C-terminal end?
    • Yes, FLAG Tag antibodies can recognize FLAG-tag at both the N- or C-terminal.
  6. Hello. We are planning to promote DYKDDDDK Antibodies in our February 2013 newsletter. Thus, we would like to check toxicity of a few products. Could you let us know whether these are lyophilized form?
    • NBP1-97391 does not come in lyophilized form.

Secondary Antibodies

 

Isotype Controls

Additional DYKDDDDK Epitope Tag Products

DYKDDDDK Epitope Tag NBP1-97391

Blogs on DYKDDDDK Epitope Tag.

Hoist the DYKDDDDK Epitope Tag
The DYKDDDDK Epitope Tag antibody was raised against a short, artificial sequence that is widely and commonly used as a protein fusion tag. It is pervasively present in a vast amount of scientific studies, and is a very powerful investigative research...  Read full blog post.

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