DYKDDDDK Epitope Tag Antibody (4C5)

Images

 
Western Blot: DYKDDDDK Epitope Tag Antibody (4C5) [NBP1-71705] - This antibody analyzed in western blot on 7 Myc/DDK tagged overexptession lysates
Immunocytochemistry/ Immunofluorescence: DYKDDDDK Epitope Tag Antibody (4C5) [NBP1-71705] - HeLa cells expressing transfected myc-DDK tagged GFAP tested in immunofluorescence at a dilution of 1:1000.
Flow Cytometry: DYKDDDDK Epitope Tag Antibody (4C5) [NBP1-71705] - HEK293T cells transfected with myc-DDK tagged PROM (red) or empty control vector (blue) and immunostaind at 1:1000 and analyzed by FACS.
Simple Western: DYKDDDDK Epitope Tag Antibody (4C5) [NBP1-71705] - Simple Western lane view shows a specific band for DYKDDDDK Epitope Tag in 0.1 mg/ml of Abro1 NT Tx lysate. This experiment was performed under reducing ...read more
Immunoprecipitation: DYKDDDDK Epitope Tag Antibody (4C5) [NBP1-71705] - Protein purification of human protein from over-expression cell lysate with anti-DDK antibody affinity column.

Product Details

Summary
Reactivity NASpecies Glossary
Applications WB, Simple Western, Flow, ICC/IF, IP
Clone
4C5
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

DYKDDDDK Epitope Tag Antibody (4C5) Summary

Immunogen
Anti-DDK monoclonal antibody is produced by immunizing mice with a synthetic peptide (DYKDDDDK) coupled to KLH.
Specificity
The anti-DDK (also known as Flag (R) ) antibody recognizes over expressed recombinant proteins containing the DDK epitope tag (DYKDDDDK) fused to either the amino- or carboxy-termini of targeted proteins in transfected mammalian cells or other expression systems.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:2000
  • Simple Western 1:250
  • Flow Cytometry 1:1000
  • Immunocytochemistry/Immunofluorescence 1:1000
  • Immunoprecipitation 1:200
Application Notes
Only recognizes n-terminal form in Simple Western.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.

Reactivity Notes

DDK epitope tag (DYKDDDDK)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Purity
Protein A or G purified

Alternate Names for DYKDDDDK Epitope Tag Antibody (4C5)

  • DDDDK epitope tag
  • DDK
  • DYKDDDDK Epitope Tag
  • DYKDDDDK tag
  • ECS epitope tag
  • ECS tag
  • Enterokinase Cleavage Site epitope tag
  • Enterokinase Cleavage Site tag
  • epitope tag flag
  • FLAG (FLAG is a trademark of Sigma Aldrich)
  • FLAG Tag
  • Ty 1 Tag
  • xxxDDDDK epitope tag

Background

This is a useful tool for the localisation and characterisation of DDDDK tagged proteins. The ECS epitope tag is commonly engineered onto the N- or C- terminus of a protein of interest so that the tagged protein can be analyzed and visualized using immunochemical methods. The recognized ECS epitope represents the enterokinase cleavage site DDDDK.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for DYKDDDDK Epitope Tag Antibody (NBP1-71705). (Showing 1 - 6 of 6 FAQ).

  1. Are the FLAG Tag antibodies validated in Simple Western?
    • Yes, we offer a 2 FLAG Tag antibodies that have been tested in Simple Western: NB600-345 and NBP1-71705.
  2. Does FLAG Tag antibodies comes in lyophilized form?
    • The following FLAG Tag antibodies come in lyophilized form: NBP1-97401, NBP1-97399, NBP1-97400, NBP1-97402.
  3. What the theoretical molecular weight for FLAG Tag antibodies?
    • The TMW of FLAG Tag antibodies is approximately 150 kDa.
  4. Will FLAG Tag antibodies detect internal FLAG tags? My FLAG sequence is sandwiched between two other protein sequences.
    • If you are applying your sample(s) to SDS-PAGE, under reducing condition and follow by Western Blot, DYKDDDDK epitope tag antibodies should be able to detect the tag region located internally. However, it depends on the property of your chimeric protein and if your tag is in the hydrophobic or hydrophilic region. Although DYKDDDDK is very hydrophilic, it may not be exposed to the surface if embedded deeply inside the protein with a high content of hydrophobic amino acids with or without covalently-linked modifications such as lipid moieties.
  5. Will FLAG Tag antibodies recognizes FLAG at the C-terminal end?
    • Yes, FLAG Tag antibodies can recognize FLAG-tag at both the N- or C-terminal.
  6. What is the immunogen sequence of this FLAG Tag antibody?
    • A synthetic sequence (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys) coupled to KLH.

Secondary Antibodies

 

Isotype Controls

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DYKDDDDK Epitope Tag NBP1-71705

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Blogs on DYKDDDDK Epitope Tag.

Hoist the DYKDDDDK Epitope Tag
The DYKDDDDK Epitope Tag antibody was raised against a short, artificial sequence that is widely and commonly used as a protein fusion tag. It is pervasively present in a vast amount of scientific studies, and is a very powerful investigative research...  Read full blog post.

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