DPH5 Overexpression Lysate (Denatured)

Images

 
DPH5 transfected lysate.
Lane 1: DPH5 transfected lysate (31.46 KDa) Lane 2: Non-transfected lysate.

Product Details

Summary
Product Discontinued
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Order Details


    • Catalog Number
      H00051611-T01
    • Availability
      Product Discontinued

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DPH5 Overexpression Lysate (Denatured) Summary

Description
Plasmid: pCMV-DPH5 full-length
Gene
DPH5

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, and 0.01% Bromophenol blue).

Lysate Details for Array

Type
Overexpression
Protein State
Denatured

Notes

Quality control test: Transient overexpression cell lysate was tested with Anti-DPH5 antibody by Western Blots.

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for DPH5 Overexpression Lysate (Denatured)

  • CGI-30
  • Diphthamide biosynthesis methyltransferase
  • diphthine synthase
  • DPH5 homolog (S. cerevisiae)
  • EC 2.1.1.98
  • HSPC143
  • MGC61450
  • NPD015
  • protein x 0011

Background

This gene encodes a component of the diphthamide synthesis pathway. Diphthamide is a post-translationally modified histidine residue found only on translation elongation factor 2. It is conserved from archaebacteria to humans, and is targeted by diphtheria toxin and Pseudomonas exotoxin A to halt cellular protein synthesis. The yeast and Chinese hamster homologs of this protein catalyze the trimethylation of the histidine residue on elongation factor 2, resulting in a diphthine moiety that is subsequently amidated to yield diphthamide. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Bioinformatics

Gene Symbol DPH5