Dopamine D2R/DRD2 Antibody

Product Discontinued

Dopamine D2R/DRD2 Antibody Summary

• Immunogen: D2 (aa272-282) covalently attached to a carrier protein.
• Epitope: Specific for Dopamine D2 Receptor (a.a. 272-282).
• Localization: Plasma membrane
• Specificity: Cross reacts 100% with D2 dopamine receptor, 70% with D2s dopamine receptor and 60% with D2L dopamine receptor. Does not cross react with D1, D3, D4 or D5 dopamine receptors.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: DRD2
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence 1:5000
  • Western Blot 1:800
• Application Notes: Detects two bands at ~48 kDa and ~51 kDa

Reactivity Notes

The immunogen sequence is highly conserved across a broad range of species.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Whole antiserum diluted with PBS. 10 mg/ml BSA.
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Unpurified
• Reconstitution Instructions: Reconstitute with 100ul sterile PBS.

Alternate Names for Dopamine D2R/DRD2 Antibody

• D(2) dopamine receptor
• D2DR
• D2R
• Dopamine D2 R
• Dopamine D2 receptor
• Dopamine D2R
• dopamine receptor D2 isoform
• dopamine receptor D2
• DRD2
• seven transmembrane helix receptor

Background

The DRD2 Dopamine Receptor is involved in the neuromodulation of appetitive behaviors. DRD2 may be linked to alcoholism and other substance-use disorders. Mutations in DRD2 in mice lead to the suppression of reward behavior with morphine and to the reduction of spontaneous movements, a phenotype resembling Parkinson's disease. Three alternatively spliced isoforms have been identified. DRD2 has been reported to be expressed in various regions of the brain, as well as in aorta, artery, eye, and spinal cord. ESTs have been isolated from normal pituitary and in brain, colon, lung, pancreas, and skeletal muscle cancer libraries.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Dopamine D2R/DRD2 Antibody (NB600-1261)(1)

Publication using NB600-1261

• Jahn K, Wieltsch C, Blumer N et al. A cell culture model for investigation of synapse influenceability: epigenetics, expression and function of gene targets important for synapse formation and preservation in SH-SY5Y neuroblastoma cells differentiated by retinoic acid J Neural Transm (Vienna) 2017-09-08 [PMID: 28887651] (ICC/IF, Human)

Bioinformatics

• Gene Symbol: DRD2
• Entrez:
  • 1813(Human)
• Uniprot:
  • NP_000786.1(Human)