DOK7 Antibody (1A9)

Images

 
Western Blot: DOK7 Antibody (1A9) [NBP2-02073] Analysis of extracts (35ug) from 9 different cell lines by usin g anti-DOK7 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: ...read more
Immunocytochemistry/ Immunofluorescence: DOK7 Antibody (1A9) [NBP2-02073] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY DOK7.
Immunohistochemistry-Paraffin: DOK7 Antibody (1A9) [NBP2-02073] - Staining of paraffin-embedded Human prostate tissue using anti-DOK7 mouse monoclonal antibody.
Flow Cytometry: DOK7 Antibody (1A9) [NBP2-02073] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-DOK7 antibody, and then analyzed by ...read more
Western Blot: DOK7 Antibody (1A9) [NBP2-02073] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DOK7 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ...read more
Immunohistochemistry-Paraffin: DOK7 Antibody (1A9) [NBP2-02073] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-DOK7 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: DOK7 Antibody (1A9) [NBP2-02073] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-DOK7 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: DOK7 Antibody (1A9) [NBP2-02073] - Staining of paraffin-embedded Human liver tissue using anti-DOK7 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: DOK7 Antibody (1A9) [NBP2-02073] - Staining of paraffin-embedded Human pancreas tissue using anti-DOK7 mouse monoclonal antibody.

Product Details

Summary
Reactivity Hu, Mu, MkSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IHC-P
Clone
1A9
Clonality
Monoclonal
Host
Mouse
Concentration
1 mg/ml

Order Details

DOK7 Antibody (1A9) Summary

Immunogen
Full length human recombinant protein of human DOK7(NP_775931) produced in HEK293T cell.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
DOK7
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Conjugate
Unconjugated
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Applications/Dilutions

Dilutions
  • Western Blot 1:500-2000
  • Flow Cytometry 1:100
  • Immunocytochemistry/Immunofluorescence 1:100
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin 1:150

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Alternate Names for DOK7 Antibody (1A9)

  • C4orf25
  • C4orf25chromosome 4 open reading frame 25
  • CMS1B
  • docking protein 7
  • DOK7
  • Dok-7
  • Downstream of tyrosine kinase 7
  • FLJ33718
  • FLJ39137
  • FLJ90556
  • protein Dok-7

Background

The protein encoded by this gene is essential for neuromuscular synaptogenesis. The protein functions in aneural activation of muscle-specific receptor kinase, which is required for postsynaptic differentiation, and in the subsequent clustering of the acetylcholine receptor in myotubes. This protein can also induce autophosphorylation of muscle-specific receptor kinase. Mutations in this gene are a cause of familial limb-girdle myasthenia autosomal recessive, which is also known as congenital myasthenic syndrome type 1B. Alternative splicing results in multiple transcript variants. [provided by RefSeq].

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for DOK7 Antibody (NBP2-02073) (0)

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Secondary Antibodies

 

Isotype Controls

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Blogs on DOK7

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Contact Information

Product PDFs

Bioinformatics

Gene Symbol DOK7
Entrez
Uniprot

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