DOK2 Antibody (1A4)

Images

 
Western Blot: DOK2 Antibody (1A4) [NBP2-00948] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DOK2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ...read more
Immunocytochemistry/ Immunofluorescence: DOK2 Antibody (1A4) [NBP2-00948] Staining of COS7 cells transiently transfected by pCMV6-ENTRY DOK2.
Flow Cytometry: DOK2 Antibody (1A4) [NBP2-00948] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-DOK2 antibody, and then analyzed by ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, ICC/IF
Clone
1A4
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

DOK2 Antibody (1A4) Summary

Immunogen
Full length human recombinant protein of human DOK2 (NP_003965) produced in HEK293T cell.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
DOK2
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Western Blot 1:2000
  • Flow Cytometry 1:100
  • Immunocytochemistry/Immunofluorescence 1:100

Reactivity Notes

Human

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for DOK2 Antibody (1A4)

  • docking protein 2
  • docking protein 2, 56kD
  • docking protein 2, 56kDa
  • Dok-2
  • Downstream of tyrosine kinase 2
  • p56(dok-2)
  • p56DOK
  • p56dok-2

Background

The protein encoded by this gene is constitutively tyrosine phosphorylated in hematopoietic progenitors isolated from chronic myelogenous leukemia (CML) patients in the chronic phase. It may be a critical substrate for p210(bcr/abl), a chimeric protein whose presence is associated with CML. This encoded protein binds p120 (RasGAP) from CML cells. [provided by RefSeq].

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for DOK2 Antibody (NBP2-00948) (0)

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol DOK2
Entrez