Dnmt3L Antibody (S117-9)

Images

 
Western Blot: Dnmt3L Antibody (S117-9) [NBP1-47614] - analysis of Human tissue lysate showing detection of Dnmt3L protein using Mouse Anti-Dnmt3L Monoclonal Antibody, Clone S117-9. Load: 15 ug protein. Block: 1.5% BSA ...read more
Immunocytochemistry/ Immunofluorescence: Dnmt3L Antibody (S117-9) [NBP1-47614] - Tissue: HaCaT cells. Species: Human. Fixation: Cold 100% methanol for 10 minutes at -20C. Primary Antibody: Mouse Anti-Dnmt3L Monoclonal ...read more
Immunohistochemistry: Dnmt3L Antibody (S117-9) [NBP1-47614] - Tissue: backskin. Species: Mouse. Fixation: Bouins Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-Dnmt3L Monoclonal Antibody at 1:100 for 1 ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IHC, IP
Clone
S117-9
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

Dnmt3L Antibody (S117-9) Summary

Immunogen
Fusion protein amino acids 1-387 of human DNMT3L
Localization
Nucleus
Specificity
Detects approx 45kDa. No cross-reactivity against DNMT3a or DNMT3B.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
DNMT3L
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1-10 ug/ml
  • Immunocytochemistry/Immunofluorescence 0.1-1.0 ug/ml
  • Immunohistochemistry
  • Immunoprecipitation 1:10-1:500
Application Notes
1 ug/ml of Dnmt3L Antibody was sufficient for detection of DNMT3L in 10 ug of COS cell transiently transfected with Flag-tagged DNMT3L lysate by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary Antibody.

Reactivity Notes

Based on homology, It is predicted to detect Mouse

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4) and 50% Glycerol
Preservative
0.09% Sodium Azide
Concentration
1 mg/ml
Purity
Protein G purified

Alternate Names for Dnmt3L Antibody (S117-9)

  • DNA (cytosine-5-)-methyltransferase 3-like
  • DNA (cytosine-5)-methyltransferase 3-like
  • human cytosine-5-methyltransferase 3-like protein, 10cytosine-5-methyltransferase 3-like protein
  • MGC1090

Background

CpG methylation is an epigenetic modification that is important for embryonic development, imprinting, and X-chromosome inactivation. Studies in mice have demonstrated that DNA methylation is required for mammalian development. This gene encodes a nuclear protein with similarity to DNA methyltransferases. This protein is not thought to function as a DNA methyltransferase as it does not contain the amino acid residues necessary for methyltransferase activity. However, this protein does stimulate de novo methylation by DNA cytosine methyltransferase 3 alpha and it is thought to be required for the establishment of maternal genomic imprints. This protein also mediates transcriptional repression through interaction with histone deacetylase 1. Alternative splicing results in two transcript variants. An additional splice variant has been described but its biological validity has not been determined.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Discover related pathways, diseases and genes to Dnmt3L Antibody (NBP1-47614). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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PTMs for Dnmt3L Antibody (NBP1-47614)

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Research Areas for Dnmt3L Antibody (NBP1-47614)

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Blogs on Dnmt3L.

The role of DNMT3B in the co-incidence of methyltransferase and tumor suppressor expression in malignancies
Epigenetics is the process of heritable change in gene activity despite alteration of the hosts DNA sequence, essentially causing a change in a phenotype without a change in the genotype of a host. To change the gene sequence without interfering w...  Read full blog post.

The role of DNMT3A in development
Epigenetics is the study of heritable change in gene activity despite alteration of the hosts DNA sequence.  Change in gene activity done independently of the DNA sequence is achieved by way of histone and DNA methylation.  Gene silencing in DNA ...  Read full blog post.

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Bioinformatics

Gene Symbol DNMT3L
Entrez
Uniprot