Western Blot: Dnmt1 Antibody [NBP1-00179] - Analysis of Dnmt1 in Jurkat cell lysate (35ug protein in RIPA buffer) using this antibody at 0.5 ug/ml. Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: Dnmt1 Antibody [NBP1-00179] - staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Predicted cross-reactivity based on sequence identity: Mouse, Rat, Canine, Bovine, Porcine. An anonymous customer has reported usage in rat (PC12 cells).
Packaging, Storage & Formulations
Store at -20C. Avoid freeze-thaw cycles.
0.5 mg/ml Tris (pH 7.3) and 0.5% BSA
0.02% Sodium Azide
Immunogen affinity purified
Alternate Names for DNMT1 Antibody
CXXC9CXXC finger protein 9
CXXC-type zinc finger protein 9
DNA (cytosine-5-)-methyltransferase 1
DNA (cytosine-5)-methyltransferase 1
DNA methyltransferase 1
DNA methyltransferase HsaI
DNA MTase HsaI
DNA (cytosine-5-)-methyltransferase 1 has a role in the establishment and regulation of tissue-specific patterns of methylated cytosine residues. Aberrant methylation patterns are associated with certain human tumors and developmental abnormalities. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
The role of DNMT3A in development Epigenetics is the study of heritable change in gene activity despite alteration of the hosts DNA sequence. Change in gene activity done independently of the DNA sequence is achieved by way of histone and DNA methylation. Gene silencing in DNA ... Read full blog post.
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