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DNAJB6 Antibody (2C11-C1) [DyLight 680]

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Summary
Product Discontinued
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    • Catalog Number
      H00010049-M01FR
    • Availability
      Product Discontinued

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DNAJB6 Antibody (2C11-C1) [DyLight 680] Summary

Immunogen
DNAJB6 (AAH00177, 1 a.a. ~ 242 a.a) full length recombinant protein with GST tag.MVDYYEVLGVQRHASPEDIKKAYRKLALKWHPDKNPENKEEAERKFKQVAEAYEVLSDAKKRDIYDKYGKEGLNGGGGGGSHFDSPFEFGFTFRNPDDVFREFFGGRDPFSFDFFEDPFEDFFGNRRGPRGSRSRGTGSFFSAFSGFPSFGSGFSSFDTGFTSFGSLGHGGLTSFSSTSFGGSGMGNFKSISTSTKMVNGRKITTKRIVENGQERVEVEEDGQLKSLTINGKEQLLRLDNK
Specificity
DNAJB6 - DnaJ (Hsp40) homolog, subfamily B, member 6
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
DNAJB6
Purity
IgG purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot
  • ELISA
  • Immunocytochemistry/Immunofluorescence

Reactivity Notes

Other species not tested.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
IgG purified

Notes

Dylight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries. This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for DNAJB6 Antibody (2C11-C1) [DyLight 680]

  • DJ4
  • DKFZp566D0824
  • DnaJ (Hsp40) homolog, subfamily B, member 6
  • dnaJ homolog subfamily B member 6
  • DnaJ
  • DnaJ-like 2 protein
  • FLJ42837
  • Heat shock protein J2
  • HHDJ1
  • HSJ2
  • HSJ-2
  • MGC1152
  • MRJMGC117297
  • MSJ1
  • MSJ-1

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Western Blot: HspA4 Antibody [NBP1-81696] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp. Lane 4: Human plasma (IgG/HSA depleted). Lane 5: Human liver tissue. Lane 6: Human tonsil tissue
Immunocytochemistry/Immunofluorescence: HspA4 Antibody [NBP1-81696] - Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & cytosol. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: HspA4 Antibody [NBP1-81696] - Staining of human testis shows nuclear and cytoplasmic positivity in cells in seminiferous ducts and Leydig cells.
(1 Publication)
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
HSP40/DNAJB1
Antibody
NBP2-38988
Western Blot: HSP40/DNAJB1 Antibody [NBP2-38988] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG
Immunocytochemistry/Immunofluorescence: HSP40/DNAJB1 Antibody [NBP2-38988] - Immunofluorescent staining of human cell line A549 shows localization to nucleus.
Immunohistochemistry-Paraffin: HSP40/DNAJB1 Antibody [NBP2-38988] - Staining in human adrenal gland and duodenum tissues using anti-DNAJB1 antibody. Corresponding DNAJB1 RNA-seq data are presented for the same tissues.
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
HSP60 Antibody
NBP1-77397
Western Blot: HSP60 Antibody [NBP1-77397] - Western blot analysis of extracts from HeLa and COS cells using NBP1-77397 Hsp60 antibody at 1:1000
Immunocytochemistry/Immunofluorescence: HSP60 Antibody [NBP1-77397] - Confocal immunofluorescent analysis of HeLa cells using Hsp60 antibody (NBP1-77397, 1:100). An Alexa Fluor 488-conjugated Goat to rabbit IgG was used as secondary antibody (green, A). Actin filaments were labeled with Alexa Fluor 568 phalloidin (red, B). DAPI was used to stain the cell nuclei (blue, C).
Immunohistochemistry: HSP60 Antibody [NBP1-77397] - IHC staining of HSP60 in human kidney carcinoma using DAB with hematoxylin counterstain.
(4 Reviews)
Species: Hu, Mu, Rt, Ha, Pm
Applications: WB, Simple Western, Flow, ICC/IF, IHC, IHC-P
HSP10/EPF Antibody
NBP2-16890
Western Blot: Hsp10 Antibody [NBP2-16890] - Sample (50 ug of whole cell lysate) A: Mouse Brain, 15% SDS PAGE gel, diluted at 1:1000.
Immunocytochemistry/Immunofluorescence: HSP10/EPF Antibody [NBP2-16890] - SKNSH cells were fixed in 2% paraformaldehyde/culture medium at 37 degrees C for 15 min. Green: HSP10 protein stained by HSP10 antibody diluted at 1:500. Blue: Hoechst 33342 staining.
Immunohistochemistry-Paraffin: HSP10/EPF Antibody [NBP2-16890] - Paraffin-embedded lung carcinoma. HSP10 antibody dilution: 1:250.
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-P
HSPA8/HSC71/Hsc70
Antibody (1B5)
NBP1-97868
Western Blot: HSPA8/HSC71/Hsc70 Antibody (1B5) [NBP1-97868] - Analysis using the Biotin conjugate of NBP1-97868. Detection of HSC70/HSP73, mAb (1B5) (biotin conjugate): Lane 1: HSC70/HSP73 (bovine), (rec); Lane 2: HSP70/HSP72 (human), (recombinant); Lane 3: 3T3 (heat shocked): Lane 4: PC-12 (heat shocked); Lane 5: CHO-K1 (heat shocked); Lane 6: GPC-16 (heat shocked); Lane 7: HeLa (heat shocked); Lane 8: Vero (heat shocked).
Immunohistochemistry-Paraffin: HSPA8/HSC71/Hsc70 Antibody (1B5) [NBP1-97868] - Analysis of human spleen tissue stained with HSC70/HSP73, mAb (1B5) at 10ug/ml.
Western Blot: HSPA8/HSC71/Hsc70 Antibody (1B5) [NBP1-97868] - Analysis of HSC70/HSP73: Lane 1: MW marker, Lane 2: HSC70/HSP73 (bovine), (recombinant) Lane 3: HSP70/HSP72 (human), (recombinant) Lane 4: 3T3, (Cell Lysate) Lane 5: PC-12, (Cell Lysate), Lane 6: CHO-K1 Cell Lysate, Lane 7: GPC-16 Cell Lysate, Lane 8: HeLa, (Cell Lysate).
(1 Review)
(10 Publications)
Species: Hu, Mu, Rt, Po, Bv, Ca, Ch, GP, Ha, Mk, Rb, Sh
Applications: WB, EM, EIA, Flow, ICC/IF, IHC, IHC-P, IP
HDJ2 Antibody
NBP1-88019
Western Blot: HDJ2 Antibody [NBP1-88019] - Analysis in HeLa cells transfected with control siRNA, target specific siRNA probe #1 and #2. Remaining relative intensity is presented. Loading control: Anti-PPIB.
Immunocytochemistry/Immunofluorescence: HDJ2 Antibody [NBP1-88019] - Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol & microtubules.
Immunohistochemistry-Paraffin: HDJ2 Antibody [NBP1-88019] - Staining of human testis shows strong cytoplasmic and nuclear positivity in cells in seminiferus ducts.
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P, KD
GRP78/HSPA5
Antibody
NBP1-06274
Western Blot: GRP78/HSPA5 Antibody [NBP1-06274] - Total protein from human HeLa and A431 cells, mouse 3T3 cells and rat PC12 cells was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 1.0 ug/ml anti-GPR78 in blocking buffer and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.
Immunocytochemistry/Immunofluorescence: GRP78/HSPA5 Antibody [NBP1-06274] - NIH-3T3 cells were fixed and permeabilized for 10 minutes using -20C MeOH. The cells were incubated with anti-GRP78/HSPA5 at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.
Flow Cytometry: GRP78/HSPA5 Antibody [NBP1-06274] - An intracellular stain was performed on HeLa with GRP78/HSPA5 Antibody NBP1-06274 and a matched isotype control. Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (SA5-10033, Thermo Fisher).
(1 Review)
(15 Publications)
Species: Hu, Mu, Rt, Ch, Dr, Sh
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Hsp90B2P Antibody
NBP1-02459
Immunohistochemistry-Paraffin: Hsp90B2P Antibody [NBP1-02459] - Staining of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 2.5 ug/ml.
Immunohistochemistry-Paraffin: Hsp90B2P Antibody [NBP1-02459] - Human kidney
Species: Hu, Mu, Rt, Ch, Dr, Pm
Applications: WB, ELISA, IHC, IHC-P
TID1 Antibody
NBP1-33387
Western Blot: TID1 Antibody [NBP1-33387] - Sample (30 ug of whole cell lysate) A: 293T B: A431 C: H1299 D: Hela E: Hep G2 F: Molt-4 G: Raji 10% SDS PAGE; antibody diluted at 1:1000.
Immunocytochemistry/Immunofluorescence: TID1 Antibody [NBP1-33387] - Analysis of methanol-fixed MCF-7, using TID1 antibody at 1:500 dilution.
Immunohistochemistry-Paraffin: TID1 Antibody [NBP1-33387] - Paraffin-embedded rat muscle. TID1 antibody [N3C3] diluted at 1:500.
Species: Hu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
BMP-3b/GDF-10
Antibody [Unconjugate
AF1543
BMP-3b/GDF-10 Antibody [Unconjugated]
(1 Publication)
Species: Hu
Applications: WB, IHC
CLPB Antibody
NBP1-83703
Western Blot: CLPB Antibody [NBP1-83703] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp
Immunohistochemistry-Paraffin: CLPB Antibody [NBP1-83703] - Staining of human kidney shows strong cytoplasmic positivity in cells in tubules.
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
Perforin Antibody
(CB5.4)
NBP1-97512
Western Blot: Perforin Antibody (CB5.4) [NBP1-97512] - Detection of perforin in the mouse T cell clone B6.1. (lane 1). Absence of perforin in the mouse fibroblast cell line NIH.3T3 (lane 2). The protein migrates as a 66 kDa species. Method: Cell extracts from the T cell clone B6.1 (2x10^6) were resolved by SDS-PAGE under reducing conditions, transferred to nitrocellulose and probed with the CB5.4 antibody at 1 ug/ml. Proteins were visualized using a peroxidase-conjugated antibody to rat IgG and a chemiluminescence detection system.
(5 Publications)
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Applications: WB, ICC/IF, IHC, IHC-Fr, IHC-P, IP
Recombinant Human
B3GALNT1 Protein
H00008706-P01
12.5% SDS-PAGE Stained with Coomassie Blue.
Species: Hu
NUCKS1 Antibody
NBP2-49365
Immunocytochemistry/Immunofluorescence: NUCKS1 Antibody [NBP2-49365] - Staining of human cell line MCF7 shows localization to nucleus & nucleoli.
Immunohistochemistry: NUCKS1 Antibody [NBP2-49365] - Staining of human prostate shows strong nuclear positivity in glandular cells.
Species: Hu
Applications: ICC/IF, IHC, IHC-P
Protamine 1
Antibody
NBP2-30949
Immunohistochemistry-Paraffin: Protamine 1 Antibody [NBP2-30949] - Staining of human testis shows high expression.
Immunohistochemistry-Paraffin: Protamine 1 Antibody [NBP2-30949] - Staining of human endometrium shows low expression as expected.
Immunohistochemistry-Paraffin: Protamine 1 Antibody [NBP2-30949] - Staining in human testis and endometrium tissues using anti-PRM1 antibody. Corresponding PRM1 RNA-seq data are presented for the same tissues.
Species: Hu
Applications: IHC, IHC-P
PMEL17/SILV
Antibody (OTI10E5)
NBP1-47974
Western Blot: PMEL17/SILV Antibody (10E5) [NBP1-47974] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PMEL17/SILV(Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PMEL17/SILV.
Immunocytochemistry/Immunofluorescence: PMEL17/SILV Antibody (10E5) [NBP1-47974] Staining of COS7 cells transiently transfected by pCMV6-ENTRY PMEL17/SILV.
Immunohistochemistry-Paraffin: PMEL17/SILV Antibody (10E5) [NBP1-47974] - Staining of paraffin-embedded prostate tissue using anti-PMEL17/SILV mouse monoclonal antibody.
Species: Hu, Rt, Ca, Pm
Applications: WB, ICC/IF, IHC, IHC-P
RPLP1 Antibody
NBP1-81293
Western Blot: RPLP1 Antibody [NBP1-81293] - Analysis in HeLa cells transfected with control siRNA, target specific siRNA probe #1 and #2. Remaining relative intensity is presented. Loading control: Anti-GAPDH.
Immunocytochemistry/Immunofluorescence: RPLP1 Antibody [NBP1-81293] - Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol & endoplasmic reticulum. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: RPLP1 Antibody [NBP1-81293] - Staining of human pancreas shows strong cytoplasmic positivity in exocrine glandular cells.
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P, KD
HSP90 alpha
Antibody
NB120-2928
Western Blot: HSP90 alpha Antibody [NB120-2928] - Analysis of 2-fold serial dilutions of HeLa cell lysate, starting at 10 ug, per well.
Immunocytochemistry/Immunofluorescence: HSP90 alpha Antibody [NB120-2928] - Analysis of Heat Shock Protein 86 (HSP86, green) in HeLa cells and NIH3T3 cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with a HSP86 polyclonal antibody, at a dilution of 1:100 for at least 1 hour at room temperature, washed with PBS, and incubated with DyLight 488 goat-anti-rabbit IgG secondary antibody at a dilution of 1:400 for 30 minutes at room temperature. Nuclei (blue) were stained with Hoechst 33342 dye.
Immunohistochemistry-Paraffin: HSP90 alpha Antibody [NB120-2928] - Both normal and cancer biopsies of deparaffinized Human breast carcinoma tissues.
(7 Publications)
Species: Hu, Mu, Rt, Pm, Rb, Sh
Applications: WB, ICC/IF, IHC, IHC-P, IP
CD77 Synthase
Antibody
NBP2-57614
Immunocytochemistry/Immunofluorescence: CD77 Synthase Antibody [NBP2-57614] - Staining of human cell line U-2 OS shows localization to nuclear bodies & mitochondria.
Species: Hu
Applications: ICC/IF
DNAJA2 Antibody
(OTI3A10)
NBP2-02996
Western Blot: DNAJA2 Antibody (3A10) [NBP2-02996] Analysis of extracts (35ug) from 9 different cell lines by using anti-DNAJA2 monoclonal antibody.
Immunocytochemistry/Immunofluorescence: DNAJA2 Antibody (3A10) [NBP2-02996] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY DNAJA2.
Immunohistochemistry-Paraffin: DNAJA2 Antibody (3A10) [NBP2-02996] - Staining of paraffin-embedded Human thyroid tissue using anti-DNAJA2 mouse monoclonal antibody.
Species: Hu, Rt, Ca, Pm
Applications: WB, Flow, ICC/IF, IHC, IHC-P

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Bioinformatics

Gene Symbol DNAJB6