DNA Primase small subunit Antibody

Images

 
Western Blot: DNA Primase small subunit Antibody [NBP1-51357] - Western blot (WB) analysis of PRIM1 (K413) pAb in extracts from Raw264.7 cells,treated with LPS.
Immunohistochemistry-Paraffin: DNA Primase small subunit Antibody [NBP1-51357] - Immunohistochemistry (IHC) analyzes of PRIM1 (K413) pAb in paraffin-embedded human breast carcinoma tissue.

Product Details

Summary
Product Discontinued
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Order Details


    • Catalog Number
      NBP1-51357
    • Availability
      Product Discontinued

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DNA Primase small subunit Antibody Summary

Immunogen
Synthetic peptide, corresponding to amino acids 400-450 of Human PRIM1.
Specificity
PRIM1 (K413) pAb detects endogenous levels of PRIM1 protein.
Clonality
Polyclonal
Host
Rabbit
Gene
PRIM1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:1000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:50-1:200
Application Notes
Western blot (WB) analysis of PRIM1 (K413) pAb in extracts from Raw264.7 cells,treated with LPS.
Immunohistochemistry (IHC) analyzes of PRIM1 (K413) pAb in paraffin-embedded human breast carcinoma tissue.
Theoretical MW
50 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Human, Mouse, Rat

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2)
Preservative
15mM Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for DNA Primase small subunit Antibody

  • DNA primase 1
  • DNA primase 49 kDa subunit
  • DNA primase small subunit
  • DNA primase subunit 48
  • EC 2.7.7
  • EC 2.7.7.-
  • MGC12308
  • p49
  • primase p49 subunit
  • primase polypeptide 1, 49kDa
  • primase, DNA, polypeptide 1 (49kDa)

Background

The replication of DNA in eukaryotic cells is carried out by a complex chromosomal replication apparatus, in which DNA polymerase alpha and primase are two key enzymatic components. Primase, which is a heterodimer of a small subunit and a large subunit, synthesizes small RNA primers for the Okazaki fragments made during discontinuous DNA replication. PRIM1 is the small, 49 kDa primase subunit.The replication of DNA in eukaryotic cells is carried out by a complex chromosomal replication apparatus, in which DNA polymerase alpha and primase are two key enzymatic components. Primase, which is a heterodimer of a small subunit and a large subunit, synthesizes small RNA primers for the Okazaki fragments made during discontinuous DNA replication. The protein encoded by this gene is the small, 49 kDa primase subunit.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

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Bioinformatics

Gene Symbol PRIM1